Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 637858..638434 Replicon chromosome
Accession NZ_AP022465
Organism Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)


Gene name shpA Uniprot ID A0A656ULU9
Locus tag JLF42_RS02995 Protein ID WP_025912099.1
Coordinates 637858..638145 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A156JBA1
Locus tag JLF42_RS03000 Protein ID WP_023293359.1
Coordinates 638132..638434 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF42_RS02965 632870..632986 + 117 Protein_574 amino acid-binding protein -
JLF42_RS02970 632979..633674 + 696 WP_023293362.1 winged helix-turn-helix domain-containing protein -
JLF42_RS02975 633821..634291 + 471 WP_008501325.1 MarR family transcriptional regulator -
JLF42_RS02980 634288..635355 + 1068 WP_100245850.1 HlyD family secretion protein -
JLF42_RS02985 635345..636421 + 1077 WP_055321639.1 DUF2955 domain-containing protein -
JLF42_RS02990 636418..637689 - 1272 WP_047747942.1 DUF445 domain-containing protein -
JLF42_RS02995 637858..638145 + 288 WP_025912099.1 BrnT family toxin Toxin
JLF42_RS03000 638132..638434 + 303 WP_023293359.1 BrnA antitoxin family protein Antitoxin
JLF42_RS03005 638465..639103 - 639 WP_055321638.1 LysE family translocator -
JLF42_RS03010 639151..639894 - 744 WP_055321637.1 AraC family transcriptional regulator -
JLF42_RS03015 640046..641416 + 1371 WP_045409223.1 NAD-dependent succinate-semialdehyde dehydrogenase -
JLF42_RS03020 641463..641783 - 321 WP_008501314.1 hypothetical protein -
JLF42_RS03025 641783..642328 - 546 WP_055321636.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11235.68 Da        Isoelectric Point: 7.4007

>T35690 WP_025912099.1 NZ_AP022465:637858-638145 [Enterobacter roggenkampii]
MPTEYEWDSNKAKSNLQKHGIRFEDAVMVFDDPYHLSVQDRYENGEFRWQTIGLVQGLLVILVAHTVRFESGGEIIRIIS
ARKADRKERSRYEHR

Download         Length: 288 bp

>T35690 NZ_AP022465:637858-638145 [Enterobacter roggenkampii]
ATGCCAACGGAGTATGAGTGGGATAGCAATAAGGCGAAAAGTAACCTGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TATGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAATTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGTCTTCTTGTTATTCTGGTCGCACATACGGTACGTTTTGAAAGCGGAGGAGAAATCATCCGTATCATTAGC
GCACGAAAGGCGGACCGCAAAGAGAGGAGCCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11369.01 Da        Isoelectric Point: 10.2483

>AT35690 WP_023293359.1 NZ_AP022465:638132-638434 [Enterobacter roggenkampii]
MSIVKHKRGSAPEVSARRVAELKALAEKPDAEIDYSDIPATDDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT35690 NZ_AP022465:638132-638434 [Enterobacter roggenkampii]
ATGAGCATCGTTAAACATAAACGCGGCAGTGCGCCTGAGGTAAGCGCTCGTCGTGTGGCTGAGCTCAAAGCATTAGCTGA
AAAACCAGATGCGGAGATTGATTACAGCGATATTCCTGCCACGGACGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGACCACTCAAAACTCAGGCATCTGTACGCATTGACGCCGATGTGATGGAGTGGCTTAAACGGCCTGGGAAAGGT
TATCAGACGCGGCTCAATGCCATTTTGCGTGAAGCAATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A656ULU9

Antitoxin

Source ID Structure
AlphaFold DB A0A156JBA1

References