Detailed information of TA system TA035686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 44216..44877
• Replicon: chromosome
• Accession: NZ_AP022465
• Organism: Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF42_RS00225
• Protein ID: WP_023293716.1
• Coordinates: 44479..44877 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A156KC23
• Locus tag: JLF42_RS00220
• Protein ID: WP_032675702.1
• Coordinates: 44216..44482 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF42_RS00195	40269..41597	-	1329	WP_023293713.1	PTS sugar transporter subunit IIC	-
JLF42_RS00200	41609..41923	-	315	WP_008500132.1	PTS sugar transporter subunit IIB	-
JLF42_RS00205	42217..43167	+	951	WP_023293714.1	LacI family transcriptional regulator	-
JLF42_RS00210	43229..43525	-	297	WP_021242704.1	hypothetical protein	-
JLF42_RS00215	43667..44086	+	420	WP_008500129.1	GNAT family N-acetyltransferase	-
JLF42_RS00220	44216..44482	+	267	WP_032675702.1	virulence protein	Antitoxin
JLF42_RS00225	44479..44877	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF42_RS00230	45017..45817	+	801	WP_008500128.1	lipoprotein NlpA	-
JLF42_RS00235	45940..46719	+	780	WP_055321085.1	MBL fold metallo-hydrolase	-
JLF42_RS00240	46746..47699	+	954	WP_055321086.1	helix-turn-helix domain-containing protein	-
JLF42_RS00245	47939..49579	+	1641	WP_200925782.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T35686 WP_023293716.1 NZ_AP022465:44479-44877 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T35686 NZ_AP022465:44479-44877 [Enterobacter roggenkampii]
ATGAGACATATGCTGGATACGAATATTGTGAGTCACTTGTTTAAACGACACCCGGAGGTTGTAAGCAGAATGACATGTCT
GGCGCCGGGTGACGTTTGTATCTCAAGCATTACCGAAGCGGAATTGCTGTATGGTGCTGACAAGAAGAAAAGCAGTAGAT
TGAAAGAAACAATCCGGGAGTTTCTCAATACCATCACGATTTGCGACTGGGACAGCGACGCCGCAGCAACGTACGGTGAA
CTCCGTGCAACCCTGGAAAAGAAAGGGAAAGTGATGGGGGATCTCGATCAACTGATCGCTGCACATGCTATCAGTCGGGG
AACAACGATTGTTACTAACGATCGTGCATTCAGAATGGTGCAGGAGCTTGCCGTCGAGGACTGGACGACAACGTTGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.34 Da
• Isoelectric Point: 4.6310

>AT35686 WP_032675702.1 NZ_AP022465:44216-44482 [Enterobacter roggenkampii]
MERTAKLFRKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLAAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT35686 NZ_AP022465:44216-44482 [Enterobacter roggenkampii]
ATGGAACGCACAGCAAAACTATTCAGAAAGGGTCAGAATCAGGCCGTTATGCTGCCTGCTGAATTCGCGTTTGATACGGA
GAGCGTCTACATCCGACGGGATGAGGAGGGGAATGTCGTTTTGGCGGCAAAACCTGTTCTAATAAAGAGCTGGGATAATT
TTCTCCGCATGCTGGCAGAAACGGACGTACCAGATACGTTTTTGAGCAAGGAGGAACGCAATCAGAGTGTTACGACAAGA
GACCCTCTGGATGGAGCATGCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A156KC23