Detailed information of TA system TA035670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 102..508
• Replicon: plasmid pSTW0522-60-4
• Accession: NZ_AP022450
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: JLF31_RS26700
• Protein ID: WP_200923986.1
• Coordinates: 102..269 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: JLF31_RS26705
• Protein ID: WP_094965907.1
• Coordinates: 269..508 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS26700	102..269	-	168	WP_200923986.1	CcdB family protein	Toxin
JLF31_RS26705	269..508	-	240	WP_094965907.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JLF31_RS26710	632..2203	-	1572	WP_094965906.1	relaxase/mobilization nuclease domain-containing protein	-
JLF31_RS26715	2184..2522	-	339	WP_094965905.1	MobC family plasmid mobilization relaxosome protein	-
JLF31_RS26720	2721..3011	+	291	WP_094965911.1	hypothetical protein	-
JLF31_RS26725	3164..4150	-	987	WP_094965910.1	replication initiation protein	-
JLF31_RS26730	4312..4743	-	432	WP_094965909.1	hypothetical protein	-
JLF31_RS26735	4858..5067	-	210	WP_200923985.1	CcdB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5650	5650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-35)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6230.12 Da
• Isoelectric Point: 8.4598

>T35670 WP_200923986.1 NZ_AP022450:c269-102 [Enterobacter kobei]
MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIGVRLEKGNYPTLSAFFAQMATD

Nucleotide

• Length: 168 bp

>T35670 NZ_AP022450:c269-102 [Enterobacter kobei]
ATGCAGTTCACGATCTACCGCAACAAGGGCAATGCCCGCGCTTACCCCTATCTGCTGGATGTGCAAAGCGACATCATCGG
CGAGCTGGCCACCAGAATAGGGGTCCGCTTGGAAAAAGGAAATTATCCTACGCTAAGCGCATTTTTTGCACAGATGGCTA
CCGACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8680.78 Da
• Isoelectric Point: 6.4656

>AT35670 WP_094965907.1 NZ_AP022450:c508-269 [Enterobacter kobei]
MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISGSEAERWKRENSAAIQELNRITEEHGLLSDDYRAF

Nucleotide

• Length: 240 bp

>AT35670 NZ_AP022450:c508-269 [Enterobacter kobei]
ATGGTCGCAGCAGCAATGCGCAGCAAGAGAACGGTCAGCGTCACGCTGGAGCCCAGCTTGCTGGAACGAGCCAGAGCGGC
GGGGATCAACCTGTCCGCCACCCTTGCAGCCGCACTACAGCATGAAATCAGCGGATCAGAAGCAGAACGCTGGAAGCGTG
AGAATAGTGCAGCCATACAGGAGCTTAACCGCATCACCGAGGAGCACGGTTTGCTTTCCGACGATTACAGGGCGTTCTGA