Detailed information of TA system TA035668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 233231..233781
• Replicon: plasmid pSTW0522-60-1
• Accession: NZ_AP022447
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: JLF31_RS25700
• Protein ID: WP_007372286.1
• Coordinates: 233473..233781 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: JLF31_RS25695
• Protein ID: WP_007372285.1
• Coordinates: 233231..233470 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS25655	228361..229056	+	696	WP_007372278.1	hypothetical protein	-
JLF31_RS25660	229079..229276	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
JLF31_RS25665	229329..229787	+	459	WP_008786599.1	hypothetical protein	-
JLF31_RS25670	229935..230075	+	141	WP_007372281.1	hypothetical protein	-
JLF31_RS25675	230091..230723	+	633	WP_007372282.1	hypothetical protein	-
JLF31_RS25680	231389..232086	-	698	Protein_256	IS1 family transposase	-
JLF31_RS25685	232134..232631	+	498	Protein_257	hypothetical protein	-
JLF31_RS25690	232628..233092	+	465	WP_008786596.1	hypothetical protein	-
JLF31_RS25695	233231..233470	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JLF31_RS25700	233473..233781	+	309	WP_007372286.1	CcdB family protein	Toxin
JLF31_RS25705	233802..233960	+	159	WP_016241558.1	hypothetical protein	-
JLF31_RS25710	234115..234669	+	555	WP_007372288.1	hypothetical protein	-
JLF31_RS25715	234739..235356	+	618	WP_007372289.1	hypothetical protein	-
JLF31_RS25720	235439..235909	+	471	WP_007372290.1	hypothetical protein	-
JLF31_RS25725	236256..236498	+	243	WP_007372291.1	hypothetical protein	-
JLF31_RS25730	236509..236907	+	399	WP_007372292.1	hypothetical protein	-
JLF31_RS25735	236991..237317	+	327	WP_007372293.1	hypothetical protein	-
JLF31_RS25740	237680..238018	-	339	WP_007372294.1	hypothetical protein	-
JLF31_RS25745	238256..238699	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..278483	278483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 13219.95 Da
• Isoelectric Point: 12.8085

>T35668 WP_007372286.1 NZ_AP022447:233473-233781 [Enterobacter kobei]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 309 bp

>T35668 NZ_AP022447:233473-233781 [Enterobacter kobei]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 10271.94 Da
• Isoelectric Point: 12.6975

>AT35668 WP_007372285.1 NZ_AP022447:233231-233470 [Enterobacter kobei]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 240 bp

>AT35668 NZ_AP022447:233231-233470 [Enterobacter kobei]
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3