Detailed information of TA system TA035665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3318712..3319818
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JLF31_RS16180
• Protein ID: WP_058691085.1
• Coordinates: 3318712..3319242 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: JLF31_RS16185
• Protein ID: WP_058691086.1
• Coordinates: 3319381..3319818 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS16155	3314537..3315469	-	933	WP_023330920.1	tRNA dihydrouridine(16) synthase DusC	-
JLF31_RS16160	3315638..3316033	+	396	WP_058691084.1	CidA/LrgA family protein	-
JLF31_RS16165	3316030..3316725	+	696	WP_014884559.1	CidB/LrgB family autolysis modulator	-
JLF31_RS16170	3316855..3317739	+	885	WP_014884560.1	cytidine deaminase	-
JLF31_RS16175	3317867..3318580	+	714	WP_032636024.1	outer membrane permeability protein SanA	-
JLF31_RS16180	3318712..3319242	+	531	WP_058691085.1	hypothetical protein	Toxin
JLF31_RS16185	3319381..3319818	+	438	WP_058691086.1	helix-turn-helix domain-containing protein	Antitoxin
JLF31_RS16190	3319856..3320866	-	1011	WP_013097952.1	galactose/methyl galactoside ABC transporter permease MglC	-
JLF31_RS16195	3320882..3322402	-	1521	WP_014884563.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
JLF31_RS16200	3322482..3323480	-	999	WP_014884564.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
JLF31_RS16205	3323776..3324798	-	1023	WP_014884565.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 19575.80 Da
• Isoelectric Point: 4.1958

>T35665 WP_058691085.1 NZ_AP022446:3318712-3319242 [Enterobacter kobei]
METLTVQAYLHEVWTDIALITFPGSENNDWNTTQLNYRTEYAINFLDYDDLHAVTLNHPVSLFFNDHGNPGWLRFIDDII
PAGASRRYWINALGISELPLSQQNFLLLKFGTMSPVGNLRIKESVPEWNEFASAKTFTVTDVIERAADFLDYAQERGAAA
GGATGAGGVDSPFDVA

Nucleotide

• Length: 531 bp

>T35665 NZ_AP022446:3318712-3319242 [Enterobacter kobei]
ATGGAAACGTTAACTGTACAGGCGTATCTACATGAAGTATGGACGGATATTGCTCTCATCACATTTCCCGGTAGTGAAAA
CAACGACTGGAACACCACGCAATTAAACTATCGCACTGAGTATGCGATTAATTTTCTGGATTATGATGATTTACACGCCG
TCACGCTGAATCACCCCGTATCACTTTTTTTTAATGACCACGGAAATCCAGGCTGGCTGCGATTCATCGACGATATCATT
CCCGCCGGCGCCAGTCGTCGCTACTGGATAAATGCGCTTGGTATCAGTGAATTACCACTCAGCCAGCAAAATTTTTTATT
GCTAAAATTTGGCACAATGTCCCCTGTTGGGAATTTACGCATTAAAGAATCCGTACCGGAATGGAATGAATTCGCAAGCG
CTAAAACGTTTACGGTTACGGACGTCATCGAGCGGGCAGCCGACTTTCTGGATTATGCCCAGGAAAGAGGCGCCGCGGCA
GGTGGCGCAACCGGAGCTGGCGGAGTGGATTCCCCCTTCGACGTTGCTTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16377.73 Da
• Isoelectric Point: 10.4647

>AT35665 WP_058691086.1 NZ_AP022446:3319381-3319818 [Enterobacter kobei]
MKIPDKTESEFLQTLARIRSANGRRHHDDAEDPDKLTQVNRQRVSPSLTRVDTRDRQDLLHAAIRDILLGKITQGEALRK
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADIINKIFKPFGLQTGLVPISKSLIASLFS

Nucleotide

• Length: 438 bp

>AT35665 NZ_AP022446:3319381-3319818 [Enterobacter kobei]
ATGAAAATACCTGATAAGACAGAGTCAGAGTTTCTGCAAACATTAGCCCGTATACGCAGTGCAAATGGAAGGAGACATCA
TGACGACGCAGAGGATCCTGACAAGTTAACGCAGGTAAACAGGCAACGCGTTTCACCAAGCTTAACACGCGTCGATACAC
GCGATCGTCAGGATCTCTTGCACGCAGCCATTCGGGACATATTGTTGGGTAAAATCACGCAAGGAGAAGCACTGAGGAAA
CTCAGGGTCGAGGTGTTGGGGCTAAAACAGGATGAATATGCAAAATTAGTCAGCGTATCCCGAAAAACATTGTCAGATGT
AGAAAATAACAGAGGCAACTATTCCGCTGACATCATTAACAAAATCTTTAAACCCTTCGGGCTTCAAACCGGGCTGGTGC
CCATTTCAAAATCGCTGATAGCCTCACTTTTCTCATAA