Detailed information of TA system TA035664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3169097..3169687
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8B2Y1G2
• Locus tag: JLF31_RS15510
• Protein ID: WP_045134957.1
• Coordinates: 3169097..3169429 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: JLF31_RS15515
• Protein ID: WP_058690818.1
• Coordinates: 3169430..3169687 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS15495	3165261..3166586	+	1326	WP_058690820.1	SidA/IucD/PvdA family monooxygenase	-
JLF31_RS15500	3166613..3168802	+	2190	WP_058690819.1	TonB-dependent siderophore receptor	-
JLF31_RS15510	3169097..3169429	-	333	WP_045134957.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JLF31_RS15515	3169430..3169687	-	258	WP_058690818.1	hypothetical protein	Antitoxin
JLF31_RS15520	3170053..3170243	-	191	Protein_3042	helix-turn-helix domain-containing protein	-
JLF31_RS15525	3171100..3172053	+	954	WP_200923628.1	hypothetical protein	-
JLF31_RS15530	3172099..3173079	+	981	WP_200923523.1	IS5 family transposase	-
JLF31_RS15535	3173137..3173238	+	102	Protein_3045	curli production assembly/transport protein CsgE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11785.63 Da
• Isoelectric Point: 10.5834

>T35664 WP_045134957.1 NZ_AP022446:c3169429-3169097 [Enterobacter kobei]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLDGAGTKTMGAIRCDQPRTI
DMAARSGKRLERIPDAVVNEVLARLDAILN

Nucleotide

• Length: 333 bp

>T35664 NZ_AP022446:c3169429-3169097 [Enterobacter kobei]
ATGGACAGAGGGGAAATTTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCTTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAACTTTGCACGTA
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACGATGGGCGCTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAGCGGCAAGCGGCTGGAACGCATACCAGACGCAGTGGTGAATGAGGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9455.96 Da
• Isoelectric Point: 4.4488

>AT35664 WP_058690818.1 NZ_AP022446:c3169687-3169430 [Enterobacter kobei]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPRYSLEELLAQCDPHAEMSDEDREWIDAPVV
GKEIL

Nucleotide

• Length: 258 bp

>AT35664 NZ_AP022446:c3169687-3169430 [Enterobacter kobei]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATAAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTTGCACAGTGCGATCCGCACGCCGAAATGAGCGACGAGGATCGGGAATGGATTGATGCGCCTGTGGTG
GGCAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A8B2Y1G2