Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1184955..1185575 Replicon chromosome
Accession NZ_AP022446
Organism Enterobacter kobei strain STW0522-60

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag JLF31_RS05855 Protein ID WP_014882802.1
Coordinates 1184955..1185173 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag JLF31_RS05860 Protein ID WP_008499288.1
Coordinates 1185201..1185575 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF31_RS05825 1180964..1181224 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
JLF31_RS05830 1181227..1181367 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JLF31_RS05835 1181364..1182074 - 711 WP_014882798.1 GNAT family N-acetyltransferase -
JLF31_RS05840 1182176..1183636 + 1461 WP_058690566.1 PLP-dependent aminotransferase family protein -
JLF31_RS05845 1183608..1184075 - 468 WP_014882800.1 membrane protein -
JLF31_RS05850 1184194..1184745 - 552 WP_014882801.1 maltose O-acetyltransferase -
JLF31_RS05855 1184955..1185173 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
JLF31_RS05860 1185201..1185575 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
JLF31_RS05865 1186200..1189346 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit -
JLF31_RS05870 1189369..1190562 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T35654 WP_014882802.1 NZ_AP022446:c1185173-1184955 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T35654 NZ_AP022446:c1185173-1184955 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT35654 WP_008499288.1 NZ_AP022446:c1185575-1185201 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT35654 NZ_AP022446:c1185575-1185201 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References