Detailed information of TA system TA035653

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 426401..427194
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: JLF31_RS02125
• Protein ID: WP_000854726.1
• Coordinates: 426401..426778 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: JLF31_RS02130
• Protein ID: WP_001548930.1
• Coordinates: 426868..427194 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS02110	424780..425622	-	843	WP_182945218.1	DUF4942 domain-containing protein	-
JLF31_RS02115	425707..425904	-	198	WP_000839269.1	DUF957 domain-containing protein	-
JLF31_RS02120	425916..426404	-	489	WP_001547769.1	hypothetical protein	-
JLF31_RS02125	426401..426778	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
JLF31_RS02130	426868..427194	-	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
JLF31_RS02135	427213..427434	-	222	WP_000691994.1	DUF987 domain-containing protein	-
JLF31_RS02140	427443..427919	-	477	WP_000811693.1	RadC family protein	-
JLF31_RS02145	427935..428405	-	471	WP_182945219.1	antirestriction protein	-
JLF31_RS02150	428479..428697	-	219	WP_182945303.1	DUF905 domain-containing protein	-
JLF31_RS02155	428740..429561	-	822	WP_167819612.1	DUF945 domain-containing protein	-
JLF31_RS02160	429663..430340	-	678	WP_137376005.1	hypothetical protein	-
JLF31_RS02165	430630..431523	-	894	WP_032666736.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T35653 WP_000854726.1 NZ_AP022446:c426778-426401 [Enterobacter kobei]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T35653 NZ_AP022446:c426778-426401 [Enterobacter kobei]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGGTTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT35653 WP_001548930.1 NZ_AP022446:c427194-426868 [Enterobacter kobei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT35653 NZ_AP022446:c427194-426868 [Enterobacter kobei]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCTTCGGCTCCTGTGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAA
AAAGTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7