Detailed information of TA system TA035652

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 66438..67157
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A144XIR2
• Locus tag: JLF31_RS00355
• Protein ID: WP_023305176.1
• Coordinates: 66438..66758 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JLF31_RS00360
• Protein ID: WP_032660963.1
• Coordinates: 66792..67157 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS00325	62276..62713	+	438	WP_058690221.1	acetyltransferase	-
JLF31_RS00330	62743..63042	-	300	WP_032635284.1	XRE family transcriptional regulator	-
JLF31_RS00335	63039..63389	-	351	WP_058690226.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF31_RS00340	63920..64261	-	342	WP_058690222.1	SymE family type I addiction module toxin	-
JLF31_RS00345	64390..64551	+	162	WP_072205328.1	DUF3950 domain-containing protein	-
JLF31_RS00350	64797..66302	+	1506	WP_058690223.1	DNA polymerase	-
JLF31_RS00355	66438..66758	-	321	WP_023305176.1	TA system toxin CbtA family protein	Toxin
JLF31_RS00360	66792..67157	-	366	WP_032660963.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF31_RS00365	67189..67410	-	222	WP_023305178.1	DUF987 family protein	-
JLF31_RS00370	67419..67889	-	471	WP_023305179.1	DNA repair protein RadC	-
JLF31_RS00375	67959..68780	-	822	WP_023305180.1	DUF945 domain-containing protein	-
JLF31_RS00380	69223..69414	-	192	WP_023305181.1	hypothetical protein	-
JLF31_RS00385	69473..70312	-	840	WP_050582604.1	hypothetical protein	-
JLF31_RS00390	70425..70859	-	435	WP_023305183.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	63039..88899	25860

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12190.77 Da
• Isoelectric Point: 5.9559

>T35652 WP_023305176.1 NZ_AP022446:c66758-66438 [Enterobacter kobei]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T35652 NZ_AP022446:c66758-66438 [Enterobacter kobei]
ATGCATATTTCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGATACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTCCTTGTGGAACGATTCGAGCTTGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCTTGGATAACATCTTTGGATGTTCATCGGGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13456.20 Da
• Isoelectric Point: 6.8421

>AT35652 WP_032660963.1 NZ_AP022446:c67157-66792 [Enterobacter kobei]
MQSTTLSGTSAENASCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFTDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT35652 NZ_AP022446:c67157-66792 [Enterobacter kobei]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGCATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTTGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
CTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
TTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Toxin

• AlphaFold DB: A0A144XIR2