Detailed information of TA system TA035638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1705201..1705998
• Replicon: chromosome
• Accession: NZ_AP022431
• Organism: Enterobacter kobei strain STW0522-51

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: JLF35_RS08095
• Protein ID: WP_176450254.1
• Coordinates: 1705474..1705998 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A2W0G3H3
• Locus tag: JLF35_RS08090
• Protein ID: WP_014883303.1
• Coordinates: 1705201..1705470 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF35_RS08060	1700821..1701321	+	501	WP_014883299.1	DedA family protein	-
JLF35_RS08065	1701425..1702666	+	1242	WP_200923401.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
JLF35_RS08070	1702701..1702928	-	228	WP_014883300.1	YccJ family protein	-
JLF35_RS08075	1702947..1703543	-	597	WP_014883301.1	NAD(P)H:quinone oxidoreductase	-
JLF35_RS08080	1703935..1704105	+	171	WP_023332656.1	general stress protein	-
JLF35_RS08085	1704222..1705139	+	918	WP_047028038.1	DMT family transporter	-
JLF35_RS08090	1705201..1705470	+	270	WP_014883303.1	DUF1778 domain-containing protein	Antitoxin
JLF35_RS08095	1705474..1705998	+	525	WP_176450254.1	GNAT family N-acetyltransferase	Toxin
JLF35_RS08100	1706072..1707394	-	1323	WP_014883305.1	pyrimidine utilization transport protein G	-
JLF35_RS08105	1707416..1707910	-	495	WP_023332659.1	pyrimidine utilization flavin reductase protein F	-
JLF35_RS08110	1707920..1708510	-	591	WP_193970071.1	malonic semialdehyde reductase	-
JLF35_RS08115	1708520..1709320	-	801	WP_193970070.1	pyrimidine utilization protein D	-
JLF35_RS08120	1709328..1709714	-	387	WP_014883309.1	pyrimidine utilization protein C	-
JLF35_RS08125	1709726..1710415	-	690	WP_193970069.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (90-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19689.59 Da
• Isoelectric Point: 8.9464

>T35638 WP_176450254.1 NZ_AP022431:1705474-1705998 [Enterobacter kobei]
IVANLTIEMFSEGSDYDFRGFDCGEPSLNAFLTEHLVRQHKGRILRGYLLKDRDHPRVLGYYTLSGSCFEKAMLPSKTQQ
RRIPYVNVPSVTLGRLAVHKDLQGKEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNEQAKRFYLKQGFIPLTGENSHSL
FFPTKSIEQLFEQA

Nucleotide

• Length: 525 bp

>T35638 NZ_AP022431:1705474-1705998 [Enterobacter kobei]
ATCGTGGCAAATCTCACGATCGAAATGTTTTCAGAGGGAAGTGATTACGATTTCAGGGGCTTCGATTGTGGTGAACCCTC
GCTAAATGCCTTCCTGACGGAACATCTGGTGCGTCAGCACAAGGGACGGATATTGCGAGGTTACCTGCTTAAAGATCGTG
ACCATCCGCGTGTGCTGGGCTATTACACGTTGTCCGGAAGCTGCTTTGAAAAAGCGATGCTTCCTTCTAAGACCCAGCAG
CGTCGGATCCCCTATGTCAACGTACCCAGTGTGACGCTGGGGCGTCTGGCTGTGCATAAAGATCTACAGGGAAAGGAGTG
GGGAACAACGCTCGTCGCACATGCTATGCGCGTTGTGTATCTGGCATCGCAGGCGGTAGGGGTACACGGAATCTTTGTTG
ATGCTCTCAATGAACAGGCAAAACGATTCTATCTGAAGCAGGGTTTTATTCCGCTGACAGGTGAAAACAGCCATTCTCTT
TTCTTCCCCACAAAATCTATCGAACAACTCTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10278.72 Da
• Isoelectric Point: 5.8836

>AT35638 WP_014883303.1 NZ_AP022431:1705201-1705470 [Enterobacter kobei]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNESSWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT35638 NZ_AP022431:1705201-1705470 [Enterobacter kobei]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAGACAATCACCCAGTTTATGGTGAACAGTGCGTCAGAACGCGCTGCTGAAGTCATCGAACAACATCGGCGTC
TCGTATTAAACGAGTCATCATGGAATGCGGTGATGGACGCCATCGACAATCCGCCAGAGCCAAATGAACGCCTGAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0G3H3