Detailed information of TA system TA035636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1095764..1096384
• Replicon: chromosome
• Accession: NZ_AP022431
• Organism: Enterobacter kobei strain STW0522-51

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A2W0G488
• Locus tag: JLF35_RS05210
• Protein ID: WP_014882802.1
• Coordinates: 1095764..1095982 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: JLF35_RS05215
• Protein ID: WP_193970247.1
• Coordinates: 1096010..1096384 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF35_RS05180	1091773..1092033	+	261	WP_010428154.1	type B 50S ribosomal protein L31	-
JLF35_RS05185	1092036..1092176	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
JLF35_RS05190	1092173..1092883	-	711	WP_193970250.1	GNAT family N-acetyltransferase	-
JLF35_RS05195	1092985..1094445	+	1461	WP_200923362.1	PLP-dependent aminotransferase family protein	-
JLF35_RS05200	1094417..1094884	-	468	WP_193970248.1	hypothetical protein	-
JLF35_RS05205	1095003..1095554	-	552	WP_014882801.1	maltose O-acetyltransferase	-
JLF35_RS05210	1095764..1095982	-	219	WP_014882802.1	hemolysin expression modulator Hha	Toxin
JLF35_RS05215	1096010..1096384	-	375	WP_193970247.1	Hha toxicity modulator TomB	Antitoxin
JLF35_RS05220	1096896..1100042	-	3147	WP_014882803.1	multidrug efflux RND transporter permease subunit	-
JLF35_RS05225	1100065..1101258	-	1194	WP_014882804.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T35636 WP_014882802.1 NZ_AP022431:c1095982-1095764 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T35636 NZ_AP022431:c1095982-1095764 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14467.21 Da
• Isoelectric Point: 4.6971

>AT35636 WP_193970247.1 NZ_AP022431:c1096384-1096010 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNNLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT35636 NZ_AP022431:c1096384-1096010 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAATCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2W0G488