Detailed information of TA system TA035635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 456374..456969
• Replicon: chromosome
• Accession: NZ_AP022431
• Organism: Enterobacter kobei strain STW0522-51

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: JLF35_RS02170
• Protein ID: WP_032637645.1
• Coordinates: 456619..456969 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A8B2XPR3
• Locus tag: JLF35_RS02165
• Protein ID: WP_032637647.1
• Coordinates: 456374..456625 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF35_RS02155	452041..455817	+	3777	WP_200923320.1	translocation/assembly module TamB	-
JLF35_RS02160	455820..456164	+	345	WP_014882267.1	gamma-glutamylcyclotransferase	-
JLF35_RS02165	456374..456625	+	252	WP_032637647.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLF35_RS02170	456619..456969	+	351	WP_032637645.1	type II toxin-antitoxin system ChpB family toxin	Toxin
JLF35_RS02175	457047..457577	-	531	WP_008501423.1	inorganic diphosphatase	-
JLF35_RS02180	457887..458843	+	957	WP_047626912.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JLF35_RS02185	458946..460448	+	1503	WP_045134407.1	sugar ABC transporter ATP-binding protein	-
JLF35_RS02190	460459..461484	+	1026	WP_014882273.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12354.33 Da
• Isoelectric Point: 6.9493

>T35635 WP_032637645.1 NZ_AP022431:456619-456969 [Enterobacter kobei]
MVKRPCFERGDIVLVGFDPANGHEQKGAGRPALVLSVSAFNQLGMTLVAPVTQGGNFARYAGFSVPLSCEEGNIQGVILV
NQIRMMDLGARLAKRIGVASDETVEDALLRLQAILS

Nucleotide

• Length: 351 bp

>T35635 NZ_AP022431:456619-456969 [Enterobacter kobei]
ATGGTGAAGCGACCTTGTTTTGAACGAGGTGATATCGTTCTCGTCGGCTTCGATCCTGCAAATGGTCATGAACAAAAGGG
CGCAGGCAGGCCCGCTTTAGTGCTATCCGTTAGCGCGTTCAACCAGCTGGGAATGACGCTGGTTGCGCCTGTTACACAGG
GGGGAAATTTTGCCCGCTATGCGGGTTTCAGTGTGCCGCTGTCTTGTGAAGAAGGGAATATTCAGGGCGTCATTCTGGTA
AACCAAATCCGAATGATGGATTTGGGCGCGCGTCTGGCAAAACGAATAGGCGTGGCATCAGATGAAACCGTAGAAGATGC
GCTTTTAAGGCTACAGGCCATCCTGAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9032.36 Da
• Isoelectric Point: 4.2517

>AT35635 WP_032637647.1 NZ_AP022431:456374-456625 [Enterobacter kobei]
MRITIKKWGNSAGMVIPGVVMKELGLKPGQSMEAQVTNNQLVLTPVSKQYSLEELLAQCDMSAPEINEQDAWGTSGPVGD
EVW

Nucleotide

• Length: 252 bp

>AT35635 NZ_AP022431:456374-456625 [Enterobacter kobei]
ATGCGTATAACCATTAAGAAATGGGGAAACAGTGCCGGTATGGTCATTCCTGGCGTAGTAATGAAAGAACTTGGCCTAAA
ACCAGGTCAGAGTATGGAGGCTCAGGTGACAAATAATCAGTTAGTGCTCACACCGGTGAGTAAACAGTACTCGCTTGAAG
AGTTGTTGGCGCAGTGTGATATGTCAGCCCCGGAAATTAACGAACAAGACGCATGGGGTACGTCAGGTCCCGTAGGTGAC
GAAGTATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XPR3