Detailed information of TA system TA035631

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4747133..4747904
• Replicon: chromosome
• Accession: NZ_AP022412
• Organism: Klebsiella quasipneumoniae strain STW0522-39

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2J4ZCW3
• Locus tag: JLF46_RS23190
• Protein ID: WP_042944193.1
• Coordinates: 4747133..4747522 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JLF46_RS23195
• Protein ID: WP_171840905.1
• Coordinates: 4747578..4747904 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF46_RS23170	4743294..4743932	-	639	WP_069135951.1	HNH endonuclease	-
JLF46_RS23175	4744112..4744981	+	870	WP_069135949.1	HNH endonuclease	-
JLF46_RS23180	4745261..4746058	-	798	WP_042944196.1	helix-turn-helix transcriptional regulator	-
JLF46_RS23185	4746201..4747046	-	846	WP_042944195.1	DUF4942 domain-containing protein	-
JLF46_RS23190	4747133..4747522	-	390	WP_042944193.1	TA system toxin CbtA family protein	Toxin
JLF46_RS23195	4747578..4747904	-	327	WP_171840905.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF46_RS23200	4747962..4748183	-	222	WP_004192297.1	DUF987 domain-containing protein	-
JLF46_RS23205	4748197..4748676	-	480	WP_042944191.1	DNA repair protein RadC	-
JLF46_RS23210	4748688..4749131	-	444	WP_042944189.1	antirestriction protein	-
JLF46_RS23215	4749211..4749459	-	249	WP_049100007.1	DUF905 domain-containing protein	-
JLF46_RS23220	4749465..4750289	-	825	WP_042944188.1	DUF945 domain-containing protein	-
JLF46_RS23225	4750832..4751464	+	633	WP_049100008.1	inovirus Gp2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4746201..4783175	36974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14418.54 Da
• Isoelectric Point: 8.9846

>T35631 WP_042944193.1 NZ_AP022412:c4747522-4747133 [Klebsiella quasipneumoniae]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T35631 NZ_AP022412:c4747522-4747133 [Klebsiella quasipneumoniae]
ATGCAAACAAAATCACCCCCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGGGCGTCTGGCAGACCCTGCTGAC
CTGCCTGCTGGAACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAGCAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGCCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12074.85 Da
• Isoelectric Point: 6.9367

>AT35631 WP_171840905.1 NZ_AP022412:c4747904-4747578 [Klebsiella quasipneumoniae]
ISEPWWGLRRSVSPCFGARLVQEGNRLHYLADRANVSGQFCDADLRHLDQAFPVLMKQLELMLTCGELNPRHQHCVTLYA
KGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 327 bp

>AT35631 NZ_AP022412:c4747904-4747578 [Klebsiella quasipneumoniae]
ATCAGCGAACCCTGGTGGGGGCTCAGACGCAGTGTCTCCCCGTGTTTCGGTGCCCGTCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGGGCGAATGTTAGCGGGCAGTTCTGTGATGCGGATTTACGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCTGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACGTTGTATGCA
AAAGGTCTGACCTGCGAGGCTGATACGCTGGGTTCGCACGGCTATGTTTATATCGCGATTTATCCCACTCCCGTCGCCAC
GGCATAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZCW3