Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4043474..4044050 Replicon chromosome
Accession NZ_AP022409
Organism Escherichia coli strain STW0522-31

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag JLE49_RS19230 Protein ID WP_001350372.1
Coordinates 4043763..4044050 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A1X0YJR8
Locus tag JLE49_RS19225 Protein ID WP_001402821.1
Coordinates 4043474..4043776 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE49_RS19205 4039165..4039677 + 513 WP_001175456.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
JLE49_RS19210 4040070..4042220 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
JLE49_RS19215 4042270..4042473 + 204 WP_000467859.1 YbdD/YjiX family protein -
JLE49_RS19220 4042484..4043440 + 957 WP_001295525.1 GTPase -
JLE49_RS19225 4043474..4043776 - 303 WP_001402821.1 BrnA antitoxin family protein Antitoxin
JLE49_RS19230 4043763..4044050 - 288 WP_001350372.1 BrnT family toxin Toxin
JLE49_RS19235 4044247..4045413 + 1167 WP_001672354.1 restriction endonuclease -
JLE49_RS19240 4045477..4047096 + 1620 WP_001029742.1 type I restriction-modification system subunit M -
JLE49_RS19245 4047086..4047805 + 720 Protein_3763 restriction endonuclease subunit S -
JLE49_RS19250 4047818..4048515 - 698 Protein_3764 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T35610 WP_001350372.1 NZ_AP022409:c4044050-4043763 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T35610 NZ_AP022409:c4044050-4043763 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTTACGGCATCGTGGTTATCCTGGTTGCGCATACCATCCGTTTCGAAAGTGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11534.04 Da        Isoelectric Point: 9.4646

>AT35610 WP_001402821.1 NZ_AP022409:c4043776-4043474 [Escherichia coli]
MSMVKHKRGTSSILDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT35610 NZ_AP022409:c4043776-4043474 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTATACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGCGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAGGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A1X0YJR8

References