Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3444043..3444661 Replicon chromosome
Accession NZ_AP022409
Organism Escherichia coli strain STW0522-31

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JLE49_RS16375 Protein ID WP_001291435.1
Coordinates 3444443..3444661 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JLE49_RS16370 Protein ID WP_000344800.1
Coordinates 3444043..3444417 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE49_RS16360 3439132..3440325 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLE49_RS16365 3440348..3443497 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
JLE49_RS16370 3444043..3444417 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JLE49_RS16375 3444443..3444661 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JLE49_RS16380 3444832..3445383 + 552 WP_000102556.1 maltose O-acetyltransferase -
JLE49_RS16385 3445499..3445969 + 471 WP_000136192.1 YlaC family protein -
JLE49_RS16390 3446133..3447683 + 1551 WP_024166621.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JLE49_RS16395 3447725..3448078 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JLE49_RS16405 3448457..3448768 + 312 WP_000409911.1 MGMT family protein -
JLE49_RS16410 3448799..3449371 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35608 WP_001291435.1 NZ_AP022409:3444443-3444661 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35608 NZ_AP022409:3444443-3444661 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35608 WP_000344800.1 NZ_AP022409:3444043-3444417 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35608 NZ_AP022409:3444043-3444417 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References