Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1325965..1326590 Replicon chromosome
Accession NZ_AP022409
Organism Escherichia coli strain STW0522-31

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag JLE49_RS06330 Protein ID WP_000911329.1
Coordinates 1326192..1326590 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag JLE49_RS06325 Protein ID WP_000450524.1
Coordinates 1325965..1326192 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE49_RS06300 1321768..1322238 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
JLE49_RS06305 1322238..1322810 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
JLE49_RS06310 1322956..1323834 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
JLE49_RS06315 1323851..1324885 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
JLE49_RS06320 1325098..1325811 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
JLE49_RS06325 1325965..1326192 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
JLE49_RS06330 1326192..1326590 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
JLE49_RS06335 1326737..1327600 + 864 WP_001267498.1 neutral zinc metallopeptidase -
JLE49_RS06340 1327615..1329630 + 2016 WP_200922269.1 tRNA(Met) cytidine acetyltransferase TmcA -
JLE49_RS06345 1329704..1330402 + 699 WP_000679823.1 esterase -
JLE49_RS06350 1330512..1330712 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T35597 WP_000911329.1 NZ_AP022409:1326192-1326590 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T35597 NZ_AP022409:1326192-1326590 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT35597 WP_000450524.1 NZ_AP022409:1325965-1326192 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT35597 NZ_AP022409:1325965-1326192 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References