35593

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3892..4426 Replicon plasmid pSTW0522-30-2
Accession NZ_AP022401
Organism Citrobacter portucalensis strain STW0522-30

Toxin (Protein)


Gene name relE Uniprot ID A0A6P1V8P2
Locus tag JLF43_RS25045 Protein ID WP_000143805.1
Coordinates 3892..4182 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7I6XZR7
Locus tag JLF43_RS25050 Protein ID WP_000104338.1
Coordinates 4172..4426 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF43_RS25025 32..109 + 78 WP_011201829.1 RepA leader peptide Tap -
JLF43_RS25030 90..965 + 876 WP_024191793.1 incFII family plasmid replication initiator RepA -
JLF43_RS25035 1954..2859 + 906 WP_032637320.1 hypothetical protein -
JLF43_RS25040 2982..3581 + 600 WP_032637323.1 DUF2913 family protein -
JLF43_RS25045 3892..4182 - 291 WP_000143805.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF43_RS25050 4172..4426 - 255 WP_000104338.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
JLF43_RS25055 4583..5293 - 711 WP_032637325.1 tyrosine-type recombinase/integrase -
JLF43_RS25060 5380..5775 - 396 WP_032637393.1 hypothetical protein -
JLF43_RS25065 6782..7762 - 981 WP_033146059.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - wzm / wzt / wbbM / glf / wbbN / wbbO 1..82100 82100
- flank IS/Tn - - 6782..7762 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-47)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11463.41 Da        Isoelectric Point: 10.2689

>T35593 WP_000143805.1 NZ_AP022401:c4182-3892 [Citrobacter portucalensis]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Download         Length: 291 bp

>T35593 NZ_AP022401:c4182-3892 [Citrobacter portucalensis]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAATCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9708.04 Da        Isoelectric Point: 4.4247

>AT35593 WP_000104338.1 NZ_AP022401:c4426-4172 [Citrobacter portucalensis]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Download         Length: 255 bp

>AT35593 NZ_AP022401:c4426-4172 [Citrobacter portucalensis]
ATGAGCACAATTCAAATCCGCGTTGATGACGAACTGAAAAAAGACGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCAGACGCACTTCGTCTGTTCCTGCGGTATGTCGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTGGTTCGGGAAAGACTGAAAAATCCGGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V8P2

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6XZR7

References