Detailed information of TA system TA035591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4809584..4810200
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF43_RS22930
• Protein ID: WP_126957449.1
• Coordinates: 4809584..4809958 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: JLF43_RS22935
• Protein ID: WP_003825279.1
• Coordinates: 4809958..4810200 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS22915	4807087..4807989	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
JLF43_RS22920	4807986..4808621	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
JLF43_RS22925	4808618..4809547	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
JLF43_RS22930	4809584..4809958	-	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF43_RS22935	4809958..4810200	-	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLF43_RS22940	4810406..4811314	+	909	WP_200920942.1	alpha/beta hydrolase	-
JLF43_RS22945	4811379..4811621	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
JLF43_RS22950	4811614..4811901	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF43_RS22955	4811907..4812848	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
JLF43_RS22960	4812893..4813330	-	438	WP_126957455.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF43_RS22965	4813327..4814199	-	873	WP_008786998.1	virulence factor BrkB family protein	-
JLF43_RS22970	4814193..4814792	-	600	WP_003825293.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T35591 WP_126957449.1 NZ_AP022399:c4809958-4809584 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T35591 NZ_AP022399:c4809958-4809584 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAATATCCTAATCGACCTGTTTAGCGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGTCGCCACAGAACGCAATCAGCCTCATTACATGGATGGAGGTAATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGCACTCGCGTTGCGCTAGGTGCTTTTAACGTGATCAGCATTTCACAGGATATCGCTGAACGAAGCGTGAGTATC
AGGCAGGAACATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGCTTCATCGTCTTACACTGGTTACGCG
TAATACCAAGGATTTTGCAGGCATATCTGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT35591 WP_003825279.1 NZ_AP022399:c4810200-4809958 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT35591 NZ_AP022399:c4810200-4809958 [Citrobacter portucalensis]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAACCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAAGATCAGGCTGAAACAG
CTATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTACGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8