Detailed information of TA system TA035590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4654320..4654908
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF43_RS22220
• Protein ID: WP_063941209.1
• Coordinates: 4654320..4654637 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: JLF43_RS22225
• Protein ID: WP_032942057.1
• Coordinates: 4654630..4654908 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS22195	4650088..4650912	+	825	WP_200920925.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JLF43_RS22200	4650981..4652204	+	1224	WP_200920926.1	zinc-binding dehydrogenase	-
JLF43_RS22205	4652206..4653018	+	813	WP_063941212.1	shikimate 5-dehydrogenase	-
JLF43_RS22210	4653068..4653373	-	306	WP_032934126.1	hypothetical protein	-
JLF43_RS22215	4653505..4654056	+	552	WP_200920927.1	AAA family ATPase	-
JLF43_RS22220	4654320..4654637	+	318	WP_063941209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF43_RS22225	4654630..4654908	+	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
JLF43_RS22230	4655019..4655708	+	690	WP_200921622.1	dipeptidase PepE	-
JLF43_RS22235	4655823..4657454	-	1632	WP_003826514.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4644310..4654908	10598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 10.1413

>T35590 WP_063941209.1 NZ_AP022399:4654320-4654637 [Citrobacter portucalensis]
IMEILQTMVFRRWEQNLRDRRAKTLIATRLFRLANGLSGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARSLDYQEVFRHE

Nucleotide

• Length: 318 bp

>T35590 NZ_AP022399:4654320-4654637 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTTTGGCTAATGGTTTATCTGGTGATGTAAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTTGCCCGTTCACTGGATTATCAGGAGGTCTTCCGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT35590 WP_032942057.1 NZ_AP022399:4654630-4654908 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT35590 NZ_AP022399:4654630-4654908 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGTGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTGGCTCGTGCTAAGGGTATGGCGAACATCGCAGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACACTTGCGGTG
ATGAAGGTGCTCGGTATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7