Detailed information of TA system TA035587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2639252..2639988
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JLF43_RS12620
• Protein ID: WP_200920428.1
• Coordinates: 2639506..2639988 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JLF43_RS12615
• Protein ID: WP_008784769.1
• Coordinates: 2639252..2639518 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS12595	2634304..2635491	+	1188	WP_200920425.1	outer membrane protein transport protein	-
JLF43_RS12600	2635729..2636358	+	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
JLF43_RS12605	2636475..2636861	-	387	WP_197384987.1	hypothetical protein	-
JLF43_RS12610	2637116..2639098	+	1983	WP_200920427.1	MBL fold metallo-hydrolase	-
JLF43_RS12615	2639252..2639518	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
JLF43_RS12620	2639506..2639988	+	483	WP_200920428.1	GNAT family N-acetyltransferase	Toxin
JLF43_RS12625	2640192..2641595	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF43_RS12630	2641624..2642256	-	633	WP_001567369.1	hypothetical protein	-
JLF43_RS12635	2642637..2642753	-	117	Protein_2483	mechanosensitive ion channel protein MscS	-
JLF43_RS12640	2643185..2643562	+	378	WP_003832962.1	hypothetical protein	-
JLF43_RS12645	2643615..2644451	+	837	WP_200920429.1	DMT family transporter	-
JLF43_RS12650	2644461..2644982	-	522	WP_191222609.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2636475..2644982	8507
	-	flank	IS/Tn	-	-	2640192..2641595	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17625.46 Da
• Isoelectric Point: 9.5814

>T35587 WP_200920428.1 NZ_AP022399:2639506-2639988 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T35587 NZ_AP022399:2639506-2639988 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT35587 WP_008784769.1 NZ_AP022399:2639252-2639518 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT35587 NZ_AP022399:2639252-2639518 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA