Detailed information of TA system TA035586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2532781..2533420
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JLF43_RS12165
• Protein ID: WP_200921558.1
• Coordinates: 2532781..2532978 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JLF43_RS12170
• Protein ID: WP_200921600.1
• Coordinates: 2533004..2533420 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS12145	2529558..2529788	-	231	WP_003020233.1	DUF2554 family protein	-
JLF43_RS12150	2529978..2530103	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
JLF43_RS12155	2530103..2531113	-	1011	WP_200921557.1	cytochrome d ubiquinol oxidase subunit II	-
JLF43_RS12160	2531113..2532516	-	1404	WP_164562763.1	cytochrome ubiquinol oxidase subunit I	-
JLF43_RS12165	2532781..2532978	+	198	WP_200921558.1	type II toxin-antitoxin system HicA family toxin	Toxin
JLF43_RS12170	2533004..2533420	+	417	WP_200921600.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JLF43_RS12175	2533513..2534922	+	1410	WP_148361941.1	PLP-dependent aminotransferase family protein	-
JLF43_RS12180	2535250..2536395	+	1146	WP_008784842.1	ABC transporter substrate-binding protein	-
JLF43_RS12185	2536412..2537428	+	1017	WP_063940776.1	ABC transporter ATP-binding protein	-
JLF43_RS12190	2537429..2538373	+	945	WP_126957589.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7440.58 Da
• Isoelectric Point: 10.9523

>T35586 WP_200921558.1 NZ_AP022399:2532781-2532978 [Citrobacter portucalensis]
VKQSEFRRWLESQGVDVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLGLQCSLPVQG

Nucleotide

• Length: 198 bp

>T35586 NZ_AP022399:2532781-2532978 [Citrobacter portucalensis]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTGACAAATGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCCGGTGATGAAATAAAAGAGGCCTTGCGAAAAGCGATCATTAAGC
AGCTTGGTTTACAGTGTTCATTACCAGTACAAGGTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15092.26 Da
• Isoelectric Point: 4.3529

>AT35586 WP_200921600.1 NZ_AP022399:2533004-2533420 [Citrobacter portucalensis]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHEALQAAQGALVTAFEFYFDDNEPIPLPSAVGTEADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLNHATKIDAVQIAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT35586 NZ_AP022399:2533004-2533420 [Citrobacter portucalensis]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTAGAAGGTGGATTTGTGGTTTCTTTCCCGGACATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGAGGCTTTGCAAGCTGCACAGGGTGCATTGGTTACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCTGCAGTAGGTACTGAAGCTGACTATGTGGAAATACCACTTAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTTCTTGAATCCAATATCACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAGAT
CACTCGCTTATTCGATTTAAATCACGCGACAAAAATCGATGCAGTGCAGATAGCTGCGCGCGCCTTAGGCAAAGAACTGT
CATTAACGATGTTGTAA