Detailed information of TA system TA035577

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1281152..1281819
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: JLF43_RS06075
• Protein ID: WP_200922842.1
• Coordinates: 1281152..1281481 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF43_RS06080
• Protein ID: WP_087934336.1
• Coordinates: 1281502..1281819 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS06055	1276550..1276786	+	237	WP_200922844.1	hypothetical protein	-
JLF43_RS06060	1276788..1277252	+	465	WP_200922843.1	hypothetical protein	-
JLF43_RS06065	1277442..1279463	-	2022	WP_154023424.1	hypothetical protein	-
JLF43_RS06070	1279548..1280780	-	1233	WP_154023425.1	integrase arm-type DNA-binding domain-containing protein	-
JLF43_RS06075	1281152..1281481	-	330	WP_200922842.1	TA system toxin CbtA family protein	Toxin
JLF43_RS06080	1281502..1281819	-	318	WP_087934336.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF43_RS06085	1281837..1282058	-	222	WP_200922841.1	DUF987 domain-containing protein	-
JLF43_RS06090	1282067..1282549	-	483	WP_200922840.1	DNA repair protein RadC	-
JLF43_RS06095	1282558..1283007	-	450	WP_200922839.1	antirestriction protein	-
JLF43_RS06100	1283107..1283346	-	240	WP_087934338.1	DUF905 domain-containing protein	-
JLF43_RS06105	1283423..1283890	-	468	WP_200922838.1	hypothetical protein	-
JLF43_RS06110	1283914..1284354	-	441	WP_097344773.1	hypothetical protein	-
JLF43_RS06115	1284354..1284590	-	237	WP_200922837.1	hypothetical protein	-
JLF43_RS06120	1284631..1285332	-	702	WP_200922836.1	WYL domain-containing protein	-
JLF43_RS06125	1285549..1286370	-	822	WP_200922835.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1270864..1304430	33566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12369.23 Da
• Isoelectric Point: 7.1987

>T35577 WP_200922842.1 NZ_AP022399:c1281481-1281152 [Citrobacter portucalensis]
MNTLPATTPQAAKLYLSPVSVWQMLLTYLLEQHYGLTLNDTPFSDETVIQEHIDAGVSLSDAVNFLVEKYGLIRIDRKGF
SWREQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T35577 NZ_AP022399:c1281481-1281152 [Citrobacter portucalensis]
ATGAATACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATATCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTGAC
ATACCTGCTGGAACAGCACTATGGCCTGACCCTGAACGACACACCATTCAGTGATGAAACCGTAATACAGGAACACATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTATCCGTATCGACCGAAAGGGATTT
TCGTGGCGAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11770.30 Da
• Isoelectric Point: 6.4639

>AT35577 WP_087934336.1 NZ_AP022399:c1281819-1281502 [Citrobacter portucalensis]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECPKLDVVFPDFISQIESMLTTGELNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT35577 NZ_AP022399:c1281819-1281502 [Citrobacter portucalensis]
ATGAGCAACACCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAG
ATTTTATCAGCCAGATAGAGTCGATGTTGACCACTGGTGAACTGAATCCCCGTCATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTATTCCACTCAACGCTGA