Detailed information of TA system TA035575

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 700230..700909
• Replicon: chromosome
• Accession: NZ_AP022399
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7I6XXA8
• Locus tag: JLF43_RS03450
• Protein ID: WP_039025449.1
• Coordinates: 700568..700909 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF43_RS03445
• Protein ID: WP_200922882.1
• Coordinates: 700230..700547 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS03405	695268..695576	+	309	WP_185666326.1	monooxygenase	-
JLF43_RS03410	695713..696585	-	873	WP_200922887.1	alpha/beta hydrolase	-
JLF43_RS03415	696962..697201	+	240	WP_200922886.1	YceI family protein	-
JLF43_RS03425	698824..699015	+	192	Protein_672	DUF905 family protein	-
JLF43_RS03430	699029..699490	+	462	WP_200922885.1	antirestriction protein	-
JLF43_RS03435	699499..699981	+	483	WP_200922884.1	DNA repair protein RadC	-
JLF43_RS03440	699990..700211	+	222	WP_200922883.1	DUF987 domain-containing protein	-
JLF43_RS03445	700230..700547	+	318	WP_200922882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF43_RS03450	700568..700909	+	342	WP_039025449.1	TA system toxin CbtA family protein	Toxin
JLF43_RS03460	701272..701778	+	507	WP_149281949.1	G/U mismatch-specific DNA glycosylase	-
JLF43_RS03465	701824..703671	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
JLF43_RS03470	704031..705776	-	1746	WP_200921149.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	669122..705776	36654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12880.79 Da
• Isoelectric Point: 9.6548

>T35575 WP_039025449.1 NZ_AP022399:700568-700909 [Citrobacter portucalensis]
MNTLPATTQRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIREHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNSVQ

Nucleotide

• Length: 342 bp

>T35575 NZ_AP022399:700568-700909 [Citrobacter portucalensis]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAGCACTATGGTCTGACCCTCAACGACACCCCGTTCAGCGATGAAACGGTTATTCGGGAACATATTA
ATGCTGGTATTACACTGGCTGATGCAGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAATTCGGTACAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11854.56 Da
• Isoelectric Point: 6.9368

>AT35575 WP_200922882.1 NZ_AP022399:700230-700547 [Citrobacter portucalensis]
MSNITWGLQRAIMPCLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35575 NZ_AP022399:700230-700547 [Citrobacter portucalensis]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCATGCCGTGCCTGGGAGCTCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7I6XXA8