Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3889..4423 Replicon plasmid pSTW0522-27-3
Accession NZ_AP022397
Organism Citrobacter portucalensis strain STW0522-27

Toxin (Protein)


Gene name relE Uniprot ID A0A6P1V8P2
Locus tag JLF33_RS25845 Protein ID WP_000143805.1
Coordinates 3889..4179 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7I6XZR7
Locus tag JLF33_RS25850 Protein ID WP_000104338.1
Coordinates 4169..4423 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF33_RS25825 31..108 + 78 WP_020323529.1 RepA leader peptide Tap -
JLF33_RS25830 89..964 + 876 WP_128350917.1 incFII family plasmid replication initiator RepA -
JLF33_RS25835 1951..2856 + 906 WP_032637320.1 hypothetical protein -
JLF33_RS25840 2979..3578 + 600 WP_032637323.1 DUF2913 family protein -
JLF33_RS25845 3889..4179 - 291 WP_000143805.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF33_RS25850 4169..4423 - 255 WP_000104338.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
JLF33_RS25855 4580..5290 - 711 WP_032637325.1 tyrosine-type recombinase/integrase -
JLF33_RS25860 5377..5772 - 396 WP_032637393.1 hypothetical protein -
JLF33_RS25865 6779..7759 - 981 WP_033146059.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - wzm / glf / wbbN / wbbO 1..82401 82401
- flank IS/Tn - - 6779..7759 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-47)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11463.41 Da        Isoelectric Point: 10.2689

>T35573 WP_000143805.1 NZ_AP022397:c4179-3889 [Citrobacter portucalensis]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Download         Length: 291 bp

>T35573 NZ_AP022397:c4179-3889 [Citrobacter portucalensis]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAATCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9708.04 Da        Isoelectric Point: 4.4247

>AT35573 WP_000104338.1 NZ_AP022397:c4423-4169 [Citrobacter portucalensis]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Download         Length: 255 bp

>AT35573 NZ_AP022397:c4423-4169 [Citrobacter portucalensis]
ATGAGCACAATTCAAATCCGCGTTGATGACGAACTGAAAAAAGACGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCAGACGCACTTCGTCTGTTCCTGCGGTATGTCGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTGGTTCGGGAAAGACTGAAAAATCCGGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V8P2

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6XZR7

References