Detailed information of TA system TA035571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4851339..4851955
• Replicon: chromosome
• Accession: NZ_AP022394
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF33_RS23270
• Protein ID: WP_126957449.1
• Coordinates: 4851581..4851955 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: JLF33_RS23265
• Protein ID: WP_003825279.1
• Coordinates: 4851339..4851581 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS23230	4846747..4847346	+	600	WP_003825293.1	glucose-1-phosphatase	-
JLF33_RS23235	4847340..4848212	+	873	WP_008786998.1	virulence factor BrkB family protein	-
JLF33_RS23240	4848209..4848646	+	438	WP_126957455.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF33_RS23245	4848691..4849632	+	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
JLF33_RS23250	4849638..4849925	-	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF33_RS23255	4849918..4850160	-	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
JLF33_RS23260	4850225..4851133	-	909	WP_200920942.1	alpha/beta hydrolase	-
JLF33_RS23265	4851339..4851581	+	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLF33_RS23270	4851581..4851955	+	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF33_RS23275	4851992..4852921	-	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
JLF33_RS23280	4852918..4853553	-	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
JLF33_RS23285	4853550..4854452	-	903	WP_003825273.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T35571 WP_126957449.1 NZ_AP022394:4851581-4851955 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T35571 NZ_AP022394:4851581-4851955 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAATATCCTAATCGACCTGTTTAGCGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGTCGCCACAGAACGCAATCAGCCTCATTACATGGATGGAGGTAATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGCACTCGCGTTGCGCTAGGTGCTTTTAACGTGATCAGCATTTCACAGGATATCGCTGAACGAAGCGTGAGTATC
AGGCAGGAACATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGCTTCATCGTCTTACACTGGTTACGCG
TAATACCAAGGATTTTGCAGGCATATCTGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT35571 WP_003825279.1 NZ_AP022394:4851339-4851581 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT35571 NZ_AP022394:4851339-4851581 [Citrobacter portucalensis]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAACCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAAGATCAGGCTGAAACAG
CTATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTACGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8