Detailed information of TA system TA035570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4702667..4703255
• Replicon: chromosome
• Accession: NZ_AP022394
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF33_RS22590
• Protein ID: WP_063941209.1
• Coordinates: 4702667..4702984 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: JLF33_RS22595
• Protein ID: WP_032942057.1
• Coordinates: 4702977..4703255 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS22565	4698435..4699259	+	825	WP_200920925.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JLF33_RS22570	4699328..4700551	+	1224	WP_200920926.1	zinc-binding dehydrogenase	-
JLF33_RS22575	4700553..4701365	+	813	WP_063941212.1	shikimate 5-dehydrogenase	-
JLF33_RS22580	4701415..4701720	-	306	WP_032934126.1	hypothetical protein	-
JLF33_RS22585	4701852..4702403	+	552	WP_200920927.1	AAA family ATPase	-
JLF33_RS22590	4702667..4702984	+	318	WP_063941209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF33_RS22595	4702977..4703255	+	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
JLF33_RS22600	4703366..4704055	+	690	WP_200921622.1	dipeptidase PepE	-
JLF33_RS22605	4704170..4705801	-	1632	WP_003826514.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 10.1413

>T35570 WP_063941209.1 NZ_AP022394:4702667-4702984 [Citrobacter portucalensis]
IMEILQTMVFRRWEQNLRDRRAKTLIATRLFRLANGLSGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARSLDYQEVFRHE

Nucleotide

• Length: 318 bp

>T35570 NZ_AP022394:4702667-4702984 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTTTGGCTAATGGTTTATCTGGTGATGTAAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTTGCCCGTTCACTGGATTATCAGGAGGTCTTCCGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT35570 WP_032942057.1 NZ_AP022394:4702977-4703255 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT35570 NZ_AP022394:4702977-4703255 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGTGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTGGCTCGTGCTAAGGGTATGGCGAACATCGCAGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACACTTGCGGTG
ATGAAGGTGCTCGGTATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7