Detailed information of TA system TA035569

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4187592..4188271
• Replicon: chromosome
• Accession: NZ_AP022394
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7I6XXA8
• Locus tag: JLF33_RS20085
• Protein ID: WP_039025449.1
• Coordinates: 4187592..4187933 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF33_RS20090
• Protein ID: WP_200922882.1
• Coordinates: 4187954..4188271 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS20065	4182725..4184470	+	1746	WP_200921149.1	DNA primase	-
JLF33_RS20070	4184830..4186677	+	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
JLF33_RS20075	4186723..4187229	-	507	WP_149281949.1	G/U mismatch-specific DNA glycosylase	-
JLF33_RS20085	4187592..4187933	-	342	WP_039025449.1	TA system toxin CbtA family protein	Toxin
JLF33_RS20090	4187954..4188271	-	318	WP_200922882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF33_RS20095	4188290..4188511	-	222	WP_200922883.1	DUF987 domain-containing protein	-
JLF33_RS20100	4188520..4189002	-	483	WP_200922884.1	DNA repair protein RadC	-
JLF33_RS20105	4189011..4189472	-	462	WP_200922885.1	antirestriction protein	-
JLF33_RS20110	4189602..4190549	-	948	WP_023356254.1	IS30 family transposase	-
JLF33_RS20115	4190611..4190766	-	156	Protein_3950	DUF905 family protein	-
JLF33_RS20120	4191147..4192354	+	1208	WP_200923048.1	IS3 family transposase	-
JLF33_RS20125	4192389..4192628	-	240	WP_200922886.1	YceI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4187592..4220193	32601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12880.79 Da
• Isoelectric Point: 9.6548

>T35569 WP_039025449.1 NZ_AP022394:c4187933-4187592 [Citrobacter portucalensis]
MNTLPATTQRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIREHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNSVQ

Nucleotide

• Length: 342 bp

>T35569 NZ_AP022394:c4187933-4187592 [Citrobacter portucalensis]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAGCACTATGGTCTGACCCTCAACGACACCCCGTTCAGCGATGAAACGGTTATTCGGGAACATATTA
ATGCTGGTATTACACTGGCTGATGCAGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAATTCGGTACAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11854.56 Da
• Isoelectric Point: 6.9368

>AT35569 WP_200922882.1 NZ_AP022394:c4188271-4187954 [Citrobacter portucalensis]
MSNITWGLQRAIMPCLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35569 NZ_AP022394:c4188271-4187954 [Citrobacter portucalensis]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCATGCCGTGCCTGGGAGCTCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7I6XXA8