Detailed information of TA system TA035567

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3592822..3593489
• Replicon: chromosome
• Accession: NZ_AP022394
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: JLF33_RS17350
• Protein ID: WP_200922842.1
• Coordinates: 3593160..3593489 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF33_RS17345
• Protein ID: WP_087934336.1
• Coordinates: 3592822..3593139 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS17300	3588271..3589092	+	822	WP_200922835.1	DUF945 domain-containing protein	-
JLF33_RS17305	3589309..3590010	+	702	WP_200922836.1	WYL domain-containing protein	-
JLF33_RS17310	3590051..3590287	+	237	WP_200922837.1	hypothetical protein	-
JLF33_RS17315	3590287..3590727	+	441	WP_097344773.1	hypothetical protein	-
JLF33_RS17320	3590751..3591218	+	468	WP_200922838.1	hypothetical protein	-
JLF33_RS17325	3591295..3591534	+	240	WP_087934338.1	DUF905 domain-containing protein	-
JLF33_RS17330	3591634..3592083	+	450	WP_200922839.1	antirestriction protein	-
JLF33_RS17335	3592092..3592574	+	483	WP_200922840.1	DNA repair protein RadC	-
JLF33_RS17340	3592583..3592804	+	222	WP_200922841.1	DUF987 domain-containing protein	-
JLF33_RS17345	3592822..3593139	+	318	WP_087934336.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF33_RS17350	3593160..3593489	+	330	WP_200922842.1	TA system toxin CbtA family protein	Toxin
JLF33_RS17355	3593861..3595093	+	1233	WP_154023425.1	integrase arm-type DNA-binding domain-containing protein	-
JLF33_RS17360	3595178..3597199	+	2022	WP_154023424.1	hypothetical protein	-
JLF33_RS17365	3597389..3597853	-	465	WP_200922843.1	hypothetical protein	-
JLF33_RS17370	3597855..3598091	-	237	WP_200922844.1	hypothetical protein	-
JLF33_RS17375	3598088..3598258	-	171	WP_200922845.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3591634..3606308	14674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12369.23 Da
• Isoelectric Point: 7.1987

>T35567 WP_200922842.1 NZ_AP022394:3593160-3593489 [Citrobacter portucalensis]
MNTLPATTPQAAKLYLSPVSVWQMLLTYLLEQHYGLTLNDTPFSDETVIQEHIDAGVSLSDAVNFLVEKYGLIRIDRKGF
SWREQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T35567 NZ_AP022394:3593160-3593489 [Citrobacter portucalensis]
ATGAATACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATATCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTGAC
ATACCTGCTGGAACAGCACTATGGCCTGACCCTGAACGACACACCATTCAGTGATGAAACCGTAATACAGGAACACATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTATCCGTATCGACCGAAAGGGATTT
TCGTGGCGAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11770.30 Da
• Isoelectric Point: 6.4639

>AT35567 WP_087934336.1 NZ_AP022394:3592822-3593139 [Citrobacter portucalensis]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECPKLDVVFPDFISQIESMLTTGELNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT35567 NZ_AP022394:3592822-3593139 [Citrobacter portucalensis]
ATGAGCAACACCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAG
ATTTTATCAGCCAGATAGAGTCGATGTTGACCACTGGTGAACTGAATCCCCGTCATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTATTCCACTCAACGCTGA