Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2512281..2513017 Replicon chromosome
Accession NZ_AP022394
Organism Citrobacter portucalensis strain STW0522-27

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag JLF33_RS12070 Protein ID WP_200920428.1
Coordinates 2512535..2513017 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID -
Locus tag JLF33_RS12065 Protein ID WP_008784769.1
Coordinates 2512281..2512547 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF33_RS12045 2507776..2508723 + 948 WP_023356254.1 IS30 family transposase -
JLF33_RS12050 2508758..2509387 + 630 WP_003832974.1 winged helix-turn-helix transcriptional regulator -
JLF33_RS12055 2509504..2509890 - 387 WP_197384987.1 hypothetical protein -
JLF33_RS12060 2510145..2512127 + 1983 WP_200920427.1 MBL fold metallo-hydrolase -
JLF33_RS12065 2512281..2512547 + 267 WP_008784769.1 DUF1778 domain-containing protein Antitoxin
JLF33_RS12070 2512535..2513017 + 483 WP_200920428.1 GNAT family N-acetyltransferase Toxin
JLF33_RS12075 2513220..2514623 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
JLF33_RS12080 2514652..2515284 - 633 WP_001567369.1 hypothetical protein -
JLF33_RS12085 2515482..2516429 + 948 WP_023356254.1 IS30 family transposase -
JLF33_RS12090 2516754..2516870 - 117 Protein_2379 mechanosensitive ion channel protein MscS -
JLF33_RS12095 2517302..2517679 + 378 WP_003832962.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2509504..2520189 10685
- inside IScluster/Tn - - 2507776..2518700 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17625.46 Da        Isoelectric Point: 9.5814

>T35566 WP_200920428.1 NZ_AP022394:2512535-2513017 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Download         Length: 483 bp

>T35566 NZ_AP022394:2512535-2513017 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10159.65 Da        Isoelectric Point: 4.6558

>AT35566 WP_008784769.1 NZ_AP022394:2512281-2512547 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Download         Length: 267 bp

>AT35566 NZ_AP022394:2512281-2512547 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References