Detailed information of TA system TA035565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2405922..2406561
• Replicon: chromosome
• Accession: NZ_AP022394
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JLF33_RS11615
• Protein ID: WP_200921558.1
• Coordinates: 2405922..2406119 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JLF33_RS11620
• Protein ID: WP_200921600.1
• Coordinates: 2406145..2406561 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS11595	2402699..2402929	-	231	WP_003020233.1	DUF2554 family protein	-
JLF33_RS11600	2403119..2403244	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
JLF33_RS11605	2403244..2404254	-	1011	WP_200921557.1	cytochrome d ubiquinol oxidase subunit II	-
JLF33_RS11610	2404254..2405657	-	1404	WP_164562763.1	cytochrome ubiquinol oxidase subunit I	-
JLF33_RS11615	2405922..2406119	+	198	WP_200921558.1	type II toxin-antitoxin system HicA family toxin	Toxin
JLF33_RS11620	2406145..2406561	+	417	WP_200921600.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JLF33_RS11625	2406654..2408063	+	1410	WP_148361941.1	PLP-dependent aminotransferase family protein	-
JLF33_RS11630	2408391..2409536	+	1146	WP_008784842.1	ABC transporter substrate-binding protein	-
JLF33_RS11635	2409553..2410569	+	1017	WP_063940776.1	ABC transporter ATP-binding protein	-
JLF33_RS11640	2410570..2411514	+	945	WP_126957589.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7440.58 Da
• Isoelectric Point: 10.9523

>T35565 WP_200921558.1 NZ_AP022394:2405922-2406119 [Citrobacter portucalensis]
VKQSEFRRWLESQGVDVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLGLQCSLPVQG

Nucleotide

• Length: 198 bp

>T35565 NZ_AP022394:2405922-2406119 [Citrobacter portucalensis]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTGACAAATGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCCGGTGATGAAATAAAAGAGGCCTTGCGAAAAGCGATCATTAAGC
AGCTTGGTTTACAGTGTTCATTACCAGTACAAGGTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15092.26 Da
• Isoelectric Point: 4.3529

>AT35565 WP_200921600.1 NZ_AP022394:2406145-2406561 [Citrobacter portucalensis]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHEALQAAQGALVTAFEFYFDDNEPIPLPSAVGTEADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLNHATKIDAVQIAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT35565 NZ_AP022394:2406145-2406561 [Citrobacter portucalensis]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTAGAAGGTGGATTTGTGGTTTCTTTCCCGGACATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGAGGCTTTGCAAGCTGCACAGGGTGCATTGGTTACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCTGCAGTAGGTACTGAAGCTGACTATGTGGAAATACCACTTAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTTCTTGAATCCAATATCACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAGAT
CACTCGCTTATTCGATTTAAATCACGCGACAAAAATCGATGCAGTGCAGATAGCTGCGCGCGCCTTAGGCAAAGAACTGT
CATTAACGATGTTGTAA