Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 965786..966492 Replicon chromosome
Accession NZ_AP022394
Organism Citrobacter portucalensis strain STW0522-27

Toxin (Protein)


Gene name ykfI Uniprot ID A0A7I6XM57
Locus tag JLF33_RS04580 Protein ID WP_060659174.1
Coordinates 966124..966492 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A7I6XGP2
Locus tag JLF33_RS04575 Protein ID WP_060659175.1
Coordinates 965786..966103 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF33_RS04550 960838..961524 + 687 WP_047738081.1 WYL domain-containing protein -
JLF33_RS04555 961597..964440 + 2844 WP_115626798.1 Ag43/Cah family autotransporter adhesin -
JLF33_RS04560 964581..965045 + 465 WP_114681874.1 antirestriction protein -
JLF33_RS04565 965054..965536 + 483 WP_060659176.1 DNA repair protein RadC -
JLF33_RS04570 965545..965766 + 222 WP_047738075.1 DUF987 domain-containing protein -
JLF33_RS04575 965786..966103 + 318 WP_060659175.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JLF33_RS04580 966124..966492 + 369 WP_060659174.1 TA system toxin CbtA family protein Toxin
JLF33_RS04585 966579..966812 + 234 WP_200921625.1 hypothetical protein -
JLF33_RS04590 966899..968080 - 1182 WP_003830857.1 pyridoxal phosphate-dependent aminotransferase -
JLF33_RS04595 968093..969544 - 1452 WP_008783827.1 Na+/H+ antiporter NhaC -
JLF33_RS04600 969698..970702 - 1005 WP_200920758.1 LacI family DNA-binding transcriptional regulator -
JLF33_RS04605 970870..971319 - 450 WP_174785083.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-111)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13861.00 Da        Isoelectric Point: 7.7001

>T35555 WP_060659174.1 NZ_AP022394:966124-966492 [Citrobacter portucalensis]
MKFQPAKNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRSGF
SSQEQAPYLTVTDILHARKACGLMNSCSYREVSNIVLSRSRQ

Download         Length: 369 bp

>T35555 NZ_AP022394:966124-966492 [Citrobacter portucalensis]
ATGAAATTTCAACCTGCAAAAAATATGCGGGCGGCGAAGCCATGCCTATCGCCCGTGACTATCTGGCAAATGTTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACGCTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACCCTGGCTAATGCTATCAACTTTCTGGTGGAGAAGTATGAGTTAGTCCGTATCGATCGCAGTGGATTT
TCCTCACAGGAACAAGCGCCATATCTGACCGTGACAGATATCCTTCATGCCAGGAAAGCATGCGGATTAATGAACAGTTG
TTCTTATCGGGAAGTAAGTAATATTGTTCTTAGCCGATCGCGTCAGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11893.33 Da        Isoelectric Point: 6.3164

>AT35555 WP_060659175.1 NZ_AP022394:965786-966103 [Citrobacter portucalensis]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASNTGQFSDAECRKLDETFPHFISQMELMLTSGELNPRHAHSVTLYH
DGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT35555 NZ_AP022394:965786-966103 [Citrobacter portucalensis]
ATGAGTAACATCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCCCGCCTTGTGCAAGAAGGGAACCGGCT
GCATTATCTGGCTGACCGGGCCAGCAACACCGGTCAATTCAGTGACGCCGAATGCCGGAAACTGGATGAAACTTTCCCGC
ACTTTATCAGCCAGATGGAATTGATGCTGACCTCCGGTGAACTGAATCCTCGTCATGCCCACAGCGTCACCCTGTACCAC
GACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGCTGCGGCTACGTATACATCGCCGTTTATCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6XM57

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6XGP2

References