Detailed information of TA system TA035551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4654336..4655075
• Replicon: chromosome
• Accession: NZ_AP022389
• Organism: Citrobacter portucalensis strain STW0522-26

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: JLF45_RS22340
• Protein ID: WP_013087269.1
• Coordinates: 4654336..4654821 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: JLF45_RS22345
• Protein ID: WP_007869691.1
• Coordinates: 4654809..4655075 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF45_RS22305	4650206..4651174	+	969	WP_200920899.1	IS5 family transposase	-
JLF45_RS22310	4651231..4651599	+	369	Protein_4374	ATP-binding protein	-
JLF45_RS22315	4651797..4652015	+	219	Protein_4375	transglycosylase	-
JLF45_RS22320	4652026..4652325	+	300	WP_050010005.1	TrbC/VirB2 family protein	-
JLF45_RS22325	4652335..4653231	+	897	Protein_4377	VirB3 family type IV secretion system protein	-
JLF45_RS22330	4653296..4654000	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLF45_RS22335	4654097..4654273	+	177	Protein_4379	IS1 family transposase	-
JLF45_RS22340	4654336..4654821	-	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
JLF45_RS22345	4654809..4655075	-	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
JLF45_RS22350	4655728..4656741	+	1014	WP_069602377.1	replication initiation protein	-
JLF45_RS22355	4657621..4658427	+	807	WP_032638095.1	DUF4225 domain-containing protein	-
JLF45_RS22360	4658402..4658728	-	327	WP_032638096.1	hypothetical protein	-
JLF45_RS22365	4658859..4659629	-	771	WP_032638097.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4578929..4686015	107086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T35551 WP_013087269.1 NZ_AP022389:c4654821-4654336 [Citrobacter portucalensis]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T35551 NZ_AP022389:c4654821-4654336 [Citrobacter portucalensis]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT35551 WP_007869691.1 NZ_AP022389:c4655075-4654809 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT35551 NZ_AP022389:c4655075-4654809 [Citrobacter portucalensis]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA