Detailed information of TA system TA035548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2591500..2592236
• Replicon: chromosome
• Accession: NZ_AP022389
• Organism: Citrobacter portucalensis strain STW0522-26

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JLF45_RS12435
• Protein ID: WP_200920428.1
• Coordinates: 2591754..2592236 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JLF45_RS12430
• Protein ID: WP_008784769.1
• Coordinates: 2591500..2591766 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF45_RS12410	2586552..2587739	+	1188	WP_200920425.1	outer membrane protein transport protein	-
JLF45_RS12415	2587977..2588606	+	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
JLF45_RS12420	2588723..2589109	-	387	WP_200920426.1	hypothetical protein	-
JLF45_RS12425	2589364..2591346	+	1983	WP_200920427.1	MBL fold metallo-hydrolase	-
JLF45_RS12430	2591500..2591766	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
JLF45_RS12435	2591754..2592236	+	483	WP_200920428.1	GNAT family N-acetyltransferase	Toxin
JLF45_RS12440	2592439..2593842	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF45_RS12445	2593871..2594503	-	633	WP_001567369.1	hypothetical protein	-
JLF45_RS12450	2594884..2595000	-	117	Protein_2440	mechanosensitive ion channel protein MscS	-
JLF45_RS12455	2595432..2595809	+	378	WP_003832962.1	hypothetical protein	-
JLF45_RS12460	2595862..2596698	+	837	WP_200920429.1	DMT family transporter	-
JLF45_RS12465	2596708..2597229	-	522	WP_191222609.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2588723..2597229	8506
	-	flank	IS/Tn	-	-	2592439..2593842	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17625.46 Da
• Isoelectric Point: 9.5814

>T35548 WP_200920428.1 NZ_AP022389:2591754-2592236 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T35548 NZ_AP022389:2591754-2592236 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT35548 WP_008784769.1 NZ_AP022389:2591500-2591766 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT35548 NZ_AP022389:2591500-2591766 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA