Detailed information of TA system TA035535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1254213..1255066
• Replicon: chromosome
• Accession: NZ_AP022379
• Organism: Bifidobacterium longum subsp. longum strain JCM 7052

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V6XWY1
• Locus tag: BLGA_RS05405
• Protein ID: WP_007054468.1
• Coordinates: 1254213..1254704 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V6XX41
• Locus tag: BLGA_RS05410
• Protein ID: WP_008783109.1
• Coordinates: 1254704..1255066 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLGA_RS05385	1249283..1250515	+	1233	WP_007056207.1	sensor histidine kinase	-
BLGA_RS05390	1250550..1251275	+	726	WP_007056206.1	response regulator transcription factor	-
BLGA_RS05395	1251315..1251956	+	642	WP_007052627.1	GNAT family N-acetyltransferase	-
BLGA_RS05400	1252046..1254151	+	2106	WP_007052626.1	DUF262 domain-containing protein	-
BLGA_RS05405	1254213..1254704	-	492	WP_007054468.1	PIN domain-containing protein	Toxin
BLGA_RS05410	1254704..1255066	-	363	WP_008783109.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLGA_RS05415	1255212..1257911	-	2700	WP_008783110.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1198926..1263735	64809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-141)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-53)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18328.91 Da
• Isoelectric Point: 4.5590

>T35535 WP_007054468.1 NZ_AP022379:c1254704-1254213 [Bifidobacterium longum subsp. longum]
MTDVSMQRILLDTNVLLDYLLHRDDHAKMAEAVMELGAKNNITLLCASLSLKDIAYLSSSAIRREFKPNESEVENFTRGF
LSSRVPWRCIEQVKEMCDIVAVDESTCDKAFSLQKRHRDFEDDLIIVAAQQSGANCVVTSDAELISHFPEYCKTPAEIVA
ALE

Nucleotide

• Length: 492 bp

>T35535 NZ_AP022379:c1254704-1254213 [Bifidobacterium longum subsp. longum]
ATGACCGACGTTTCCATGCAGCGTATTTTGCTCGATACCAACGTACTGTTGGACTATTTGCTCCATCGCGATGATCACGC
CAAGATGGCGGAAGCGGTTATGGAACTTGGCGCGAAGAACAACATTACGTTGTTGTGCGCGTCATTGTCGCTGAAGGATA
TCGCATACCTATCATCATCGGCGATTCGTAGGGAATTCAAACCGAATGAATCGGAAGTGGAGAATTTCACTCGCGGCTTT
CTGTCGTCACGGGTGCCATGGCGTTGTATCGAGCAGGTGAAGGAGATGTGTGACATCGTCGCTGTCGATGAGTCGACATG
TGATAAGGCTTTTTCCCTGCAAAAACGGCATCGGGATTTTGAAGATGATCTGATCATCGTTGCCGCGCAACAATCCGGTG
CCAACTGTGTGGTCACATCGGATGCCGAATTAATCAGTCATTTTCCCGAATATTGCAAAACACCGGCTGAAATCGTGGCG
GCGCTGGAATAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13974.03 Da
• Isoelectric Point: 5.0733

>AT35535 WP_008783109.1 NZ_AP022379:c1255066-1254704 [Bifidobacterium longum subsp. longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREEEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDKMYDEQLDAYYNMKRAD

Nucleotide

• Length: 363 bp

>AT35535 NZ_AP022379:c1255066-1254704 [Bifidobacterium longum subsp. longum]
ATGACCATGGTCCAAATGAACGTTCGCATTGATGCGAAACTGAAGAACGAGGTCGAAGAAGTCCTCAAGGGCAAAGGCGT
ATCGGTGAGTGACGTAATCCGGTCGCTGTGGTTGTACATCGCCGACCGCAAGGAAGTGCCGGCATTGGAAACGACCGCCG
AAGAGCAAGCCCGCGAGGAGGAAAAGCAGCGCAAGCTGAAACTGATTCGAGAAGGTGCCGGATACGTACACAAGGAACTG
GTCAAGGCCGGACTGATCTCAGAAGACACCGACCTCATGGAAGGCCTTACCTACAAGCAGTTCCGCGACAAAATGTACGA
CGAGCAACTTGATGCGTATTACAACATGAAGAGGGCCGATTGA

Structures

Toxin

• AlphaFold DB: V6XWY1

Antitoxin

• AlphaFold DB: V6XX41