Detailed information of TA system TA035534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 1061303..1061869
• Replicon: chromosome
• Accession: NZ_AP022379
• Organism: Bifidobacterium longum subsp. longum strain JCM 7052

Toxin (Protein)

• Gene name: relE
• Uniprot ID: W6EX94
• Locus tag: BLGA_RS04510
• Protein ID: WP_003831807.1
• Coordinates: 1061303..1061596 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3BGP0
• Locus tag: BLGA_RS04515
• Protein ID: WP_007056990.1
• Coordinates: 1061600..1061869 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BLGA_RS04475	1056544..1056957	-	414	Protein_864	moaA protein	-
BLGA_RS04480	1057072..1057836	-	765	WP_012472066.1	IS21-like element ISBlo1 family helper ATPase IstB	-
BLGA_RS04485	1057833..1059356	-	1524	WP_200764690.1	IS21 family transposase	-
BLGA_RS04490	1059551..1059784	-	234	Protein_867	moaA protein	-
BLGA_RS04495	1059998..1060177	+	180	WP_007056286.1	antitoxin VbhA family protein	-
BLGA_RS04500	1060174..1060758	+	585	WP_007056278.1	Fic family protein	-
BLGA_RS04505	1060961..1061245	-	285	WP_007056282.1	hypothetical protein	-
BLGA_RS04510	1061303..1061596	-	294	WP_003831807.1	type II toxin-antitoxin system YafQ family toxin	Toxin
BLGA_RS04515	1061600..1061869	-	270	WP_007056990.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BLGA_RS04520	1062054..1063223	-	1170	WP_080824247.1	ABC transporter permease	-
BLGA_RS04525	1063220..1063900	-	681	WP_003829413.1	ABC transporter ATP-binding protein	-
BLGA_RS04530	1063897..1064787	-	891	WP_007056280.1	hypothetical protein	-
BLGA_RS04535	1064784..1065299	-	516	WP_032685213.1	hypothetical protein	-
BLGA_RS04540	1065425..1066120	+	696	WP_119107335.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1039876..1076170	36294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-87)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11218.98 Da
• Isoelectric Point: 9.7881

>T35534 WP_003831807.1 NZ_AP022379:c1061596-1061303 [Bifidobacterium longum subsp. longum]
MLKPDYTAAFQRDIKKLKRKHTDLRPLKEVIRLVLEDTADSKEVLRRRHRAHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGK

Nucleotide

• Length: 294 bp

>T35534 NZ_AP022379:c1061596-1061303 [Bifidobacterium longum subsp. longum]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAAGCACACTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTTCTTGAGGACACGGCGGACTCCAAGGAAGTATTGAGGCGTAGGCATCGCGCCCACACAC
TTACCGGTGATTTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTACTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9844.16 Da
• Isoelectric Point: 4.5742

>AT35534 WP_007056990.1 NZ_AP022379:c1061869-1061600 [Bifidobacterium longum subsp. longum]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTIVPPETMEALDEAKNRLADGQPRFNSA
DEMFDSLGI

Nucleotide

• Length: 270 bp

>AT35534 NZ_AP022379:c1061869-1061600 [Bifidobacterium longum subsp. longum]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
TCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Structures

Toxin

• AlphaFold DB: W6EX94

Antitoxin

• AlphaFold DB: I3BGP0