Detailed information of TA system TA035533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4655854..4656442
• Replicon: chromosome
• Accession: NZ_AP022378
• Organism: Citrobacter portucalensis strain IOMTU157

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IOMTU157_RS22350
• Protein ID: WP_063941209.1
• Coordinates: 4655854..4656171 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: IOMTU157_RS22355
• Protein ID: WP_032942057.1
• Coordinates: 4656164..4656442 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU157_RS22320	4651275..4651466	+	192	WP_164563246.1	HAD hydrolase family protein	-
IOMTU157_RS22325	4651453..4652211	+	759	WP_032942078.1	DeoR/GlpR family DNA-binding transcription regulator	-
IOMTU157_RS22330	4652285..4652557	+	273	WP_003826541.1	DUF3811 domain-containing protein	-
IOMTU157_RS22335	4652554..4653429	-	876	WP_008786901.1	23S rRNA pseudouridine(2604) synthase RluF	-
IOMTU157_RS22340	4653899..4654690	+	792	Protein_4380	sugar-binding transcriptional regulator	-
IOMTU157_RS22345	4654745..4655442	+	698	Protein_4381	IS1 family transposase	-
IOMTU157_RS22350	4655854..4656171	+	318	WP_063941209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IOMTU157_RS22355	4656164..4656442	+	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
IOMTU157_RS22360	4656553..4657242	+	690	WP_032942056.1	dipeptidase PepE	-
IOMTU157_RS22365	4657357..4658988	-	1632	WP_003826514.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4647355..4656442	9087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 10.1413

>T35533 WP_063941209.1 NZ_AP022378:4655854-4656171 [Citrobacter portucalensis]
IMEILQTMVFRRWEQNLRDRRAKTLIATRLFRLANGLSGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARSLDYQEVFRHE

Nucleotide

• Length: 318 bp

>T35533 NZ_AP022378:4655854-4656171 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTTTGGCTAATGGTTTATCTGGTGATGTAAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTTGCCCGTTCACTGGATTATCAGGAGGTCTTCCGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT35533 WP_032942057.1 NZ_AP022378:4656164-4656442 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT35533 NZ_AP022378:4656164-4656442 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGTGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTGGCTCGTGCTAAGGGTATGGCGAACATCGCAGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACACTTGCAGTG
ATGAAGGTGCTCGGTATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7