Detailed information of TA system TA035532

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 4327189..4327997
• Replicon: chromosome
• Accession: NZ_AP022378
• Organism: Citrobacter portucalensis strain IOMTU157

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: IOMTU157_RS20840
• Protein ID: WP_163183673.1
• Coordinates: 4327189..4327566 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: IOMTU157_RS20845
• Protein ID: WP_164563175.1
• Coordinates: 4327638..4327997 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU157_RS20815	4323424..4324296	+	873	WP_126957117.1	HNH endonuclease	-
IOMTU157_RS20820	4324477..4325465	-	989	Protein_4084	restriction endonuclease	-
IOMTU157_RS20825	4325553..4326401	-	849	WP_163183667.1	DUF4942 domain-containing protein	-
IOMTU157_RS20830	4326483..4326686	-	204	WP_163183669.1	DUF957 domain-containing protein	-
IOMTU157_RS20835	4326716..4327192	-	477	WP_163183671.1	hypothetical protein	-
IOMTU157_RS20840	4327189..4327566	-	378	WP_163183673.1	TA system toxin CbtA family protein	Toxin
IOMTU157_RS20845	4327638..4327997	-	360	WP_164563175.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IOMTU157_RS20850	4328020..4328241	-	222	WP_163183676.1	DUF987 domain-containing protein	-
IOMTU157_RS20855	4328253..4328738	-	486	WP_163183697.1	DNA repair protein RadC	-
IOMTU157_RS20860	4328791..4329261	-	471	WP_163183678.1	antirestriction protein	-
IOMTU157_RS20865	4329333..4330154	-	822	WP_163183687.1	DUF945 domain-containing protein	-
IOMTU157_RS20870	4330324..4330602	-	279	WP_175310259.1	hypothetical protein	-
IOMTU157_RS20875	4330848..4331399	-	552	WP_163183689.1	hypothetical protein	-
IOMTU157_RS20880	4331705..4332460	-	756	WP_163183701.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13940.87 Da
• Isoelectric Point: 7.3208

>T35532 WP_163183673.1 NZ_AP022378:c4327566-4327189 [Citrobacter portucalensis]
MQTQPEPHRRATRPCPSPVSIWQMLLTYLLEQHYGLTLNDTPFSDEQVIAQHIDAGISLADALNFIVEKYDLVRTDRPGF
SIQHQSPFISAIDILRARKVTGLMQRTGYKAVTCAISGQSSGGQQ

Nucleotide

• Length: 378 bp

>T35532 NZ_AP022378:c4327566-4327189 [Citrobacter portucalensis]
ATGCAGACTCAACCCGAACCCCACAGGCGGGCGACCCGGCCATGCCCGTCACCCGTCAGCATCTGGCAAATGCTGCTCAC
ATATCTGCTGGAACAACACTACGGTCTTACACTTAACGATACTCCGTTTAGTGATGAACAGGTCATTGCACAGCATATTG
ACGCCGGTATCTCACTGGCGGATGCGCTGAACTTTATAGTCGAAAAATATGACTTGGTACGCACCGACCGACCCGGTTTC
TCCATTCAACATCAGTCTCCGTTCATCAGCGCTATTGACATACTCCGGGCCAGAAAGGTCACCGGCCTGATGCAACGTAC
TGGCTATAAAGCGGTGACTTGCGCCATCAGTGGACAGTCTTCCGGGGGGCAGCAATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13264.07 Da
• Isoelectric Point: 6.3627

>AT35532 WP_164563175.1 NZ_AP022378:c4327997-4327638 [Citrobacter portucalensis]
MQNTTPVANHDIAEPWWGLKPAVTPCFGARLVQENNRLHYLTDRASITGQFSNADLHHLDQAFPVLLKQLEFMLVSGELS
PHHQHCITLYAKGLTCDADSLASCGYVYIAIYPTPARTE

Nucleotide

• Length: 360 bp

>AT35532 NZ_AP022378:c4327997-4327638 [Citrobacter portucalensis]
ATGCAGAACACAACACCTGTAGCAAACCATGATATTGCCGAACCCTGGTGGGGACTTAAACCCGCCGTCACGCCGTGCTT
TGGTGCACGGCTGGTACAGGAAAACAACCGGCTGCACTATCTCACTGACCGCGCCAGTATCACCGGACAGTTCAGCAACG
CGGATTTACACCATCTCGACCAGGCATTCCCGGTACTGCTCAAACAACTGGAATTTATGCTTGTTTCCGGTGAGCTCAGT
CCCCACCATCAGCACTGTATCACGTTATACGCAAAAGGGCTCACCTGCGACGCTGACTCGCTTGCCAGTTGTGGTTATGT
CTATATAGCGATATACCCGACACCAGCCAGAACAGAATAA