Detailed information of TA system TA035531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4262953..4263529
• Replicon: chromosome
• Accession: NZ_AP022378
• Organism: Citrobacter portucalensis strain IOMTU157

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: IOMTU157_RS20525
• Protein ID: WP_164563155.1
• Coordinates: 4263242..4263529 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: IOMTU157_RS20520
• Protein ID: WP_128612219.1
• Coordinates: 4262953..4263255 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU157_RS20500	4258490..4259032	+	543	WP_164563153.1	hypothetical protein	-
IOMTU157_RS20505	4259322..4261472	+	2151	WP_003830033.1	pyruvate/proton symporter BtsT	-
IOMTU157_RS20510	4261674..4261868	+	195	WP_003830038.1	YbdD/YjiX family protein	-
IOMTU157_RS20515	4261881..4262837	+	957	WP_164563154.1	GTPase	-
IOMTU157_RS20520	4262953..4263255	-	303	WP_128612219.1	BrnA antitoxin family protein	Antitoxin
IOMTU157_RS20525	4263242..4263529	-	288	WP_164563155.1	BrnT family toxin	Toxin
IOMTU157_RS20530	4263800..4264714	-	915	WP_164563156.1	restriction endonuclease	-
IOMTU157_RS20535	4264913..4268422	+	3510	WP_164563157.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11205.59 Da
• Isoelectric Point: 7.5237

>T35531 WP_164563155.1 NZ_AP022378:c4263529-4263242 [Citrobacter portucalensis]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHMSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGVEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T35531 NZ_AP022378:c4263529-4263242 [Citrobacter portucalensis]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTGTTCGCTTTGAGGACGCAGT
ATTGGTTTTCGACGATCCACGGCATATGTCCAGACAGGATCGTTATGAGAACGGTGAGTATCGGTGGCAGACCATTGGTC
TTGTTCATGGCATTGTGGTTATTTTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCGTTGAAGTCATTCGTATCATCAGT
GCCAGAAAGGCAGACAGTAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11486.04 Da
• Isoelectric Point: 9.5646

>AT35531 WP_128612219.1 NZ_AP022378:c4263255-4262953 [Citrobacter portucalensis]
MSMVKHKRGSVSALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT35531 NZ_AP022378:c4263255-4262953 [Citrobacter portucalensis]
ATGAGCATGGTTAAACATAAACGCGGCAGCGTATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
TAAACCGGATGATGATATTGATTACAGCGACATTCCCGTGACCGAAGATGAGCAGTGGTCAGAGGCTAATCGCGGTAAGT
TTTTCCGTCCGTTGAAAACGCAGGCATCGGTGCGCATTGATGCCGATGTGATGGAGTGGCTAAAACGCCCCGGGAAGGGT
TACCAGACGCGGCTGAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG