Detailed information of TA system TA035529

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3861409..3862115
• Replicon: chromosome
• Accession: NZ_AP022378
• Organism: Citrobacter portucalensis strain IOMTU157

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: IOMTU157_RS18625
• Protein ID: WP_164563076.1
• Coordinates: 3861409..3861777 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: IOMTU157_RS18630
• Protein ID: WP_125289438.1
• Coordinates: 3861798..3862115 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IOMTU157_RS18605	3856698..3857033	+	336	Protein_3651	GNAT family N-acetyltransferase	-
IOMTU157_RS18610	3857202..3858206	+	1005	WP_164563075.1	LacI family DNA-binding transcriptional regulator	-
IOMTU157_RS18615	3858360..3859811	+	1452	WP_003830858.1	Na+/H+ antiporter NhaC	-
IOMTU157_RS18620	3859824..3861005	+	1182	WP_003830857.1	pyridoxal phosphate-dependent aminotransferase	-
IOMTU157_RS18625	3861409..3861777	-	369	WP_164563076.1	TA system toxin CbtA family protein	Toxin
IOMTU157_RS18630	3861798..3862115	-	318	WP_125289438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
IOMTU157_RS18635	3862133..3862354	-	222	WP_164563077.1	DUF987 domain-containing protein	-
IOMTU157_RS18640	3862363..3862839	-	477	WP_164563078.1	RadC family protein	-
IOMTU157_RS18645	3862855..3863313	-	459	WP_164563079.1	antirestriction protein	-
IOMTU157_RS18650	3863410..3863649	-	240	WP_164563080.1	DUF905 domain-containing protein	-
IOMTU157_RS18655	3863727..3864199	-	473	Protein_3661	hypothetical protein	-
IOMTU157_RS18660	3864221..3864916	-	696	WP_164563081.1	hypothetical protein	-
IOMTU157_RS18665	3865099..3865800	-	702	WP_130235807.1	WYL domain-containing protein	-
IOMTU157_RS18670	3866017..3866838	-	822	WP_164563082.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3855390..3920509	65119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (4-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13725.09 Da
• Isoelectric Point: 9.1662

>T35529 WP_164563076.1 NZ_AP022378:c3861777-3861409 [Citrobacter portucalensis]
MKNLPATISRAAKPCLSPIAVWKMLLTRLLEQHYGLMLSDTPFSDEIVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQLQAPYLTATDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T35529 NZ_AP022378:c3861777-3861409 [Citrobacter portucalensis]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCATTGCAGTCTGGAAAATGTTACTTAC
TCGTCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAATCGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGTTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11767.38 Da
• Isoelectric Point: 6.6236

>AT35529 WP_125289438.1 NZ_AP022378:c3862115-3861798 [Citrobacter portucalensis]
MSNPTWGLQRDITPCLGARLVQEGNRLHYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35529 NZ_AP022378:c3862115-3861798 [Citrobacter portucalensis]
ATGAGCAACCCCACATGGGGCCTGCAGCGGGATATCACGCCGTGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCAGCGCTAA