Detailed information of TA system TA035515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 9898..10373
• Replicon: plasmid pBML2531
• Accession: NZ_AP022376
• Organism: Providencia rettgeri strain BML2531

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: G7Y73_RS21865
• Protein ID: WP_096865196.1
• Coordinates: 10092..10373 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A0U3UH28
• Locus tag: G7Y73_RS21860
• Protein ID: WP_048609183.1
• Coordinates: 9898..10089 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y73_RS21815	4977..5252	+	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
G7Y73_RS21820	5280..5705	+	426	WP_000522996.1	organomercurial transporter MerC	-
G7Y73_RS21825	5744..7429	+	1686	WP_040154008.1	mercury(II) reductase	-
G7Y73_RS21830	7447..7812	+	366	WP_001277466.1	mercury resistance co-regulator MerD	-
G7Y73_RS21835	7809..8045	+	237	WP_001087807.1	broad-spectrum mercury transporter MerE	-
G7Y73_RS21845	8111..8323	+	213	WP_000993245.1	DUF3330 domain-containing protein	-
G7Y73_RS21850	8522..9226	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
G7Y73_RS21855	9273..9437	-	165	WP_164565732.1	AAA family ATPase	-
G7Y73_RS21860	9898..10089	+	192	WP_048609183.1	DUF5397 family protein	Antitoxin
G7Y73_RS21865	10092..10373	+	282	WP_096865196.1	virulence factor	Toxin
G7Y73_RS21870	10687..11436	+	750	WP_164565733.1	replication initiation protein	-
G7Y73_RS21875	11405..12580	-	1176	Protein_16	Tn3-like element ISShes11 family transposase	-
G7Y73_RS21880	12645..13349	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
G7Y73_RS21885	13403..13837	+	435	Protein_18	Mu transposase C-terminal domain-containing protein	-
G7Y73_RS21890	13840..14700	+	861	WP_114261019.1	TniB family NTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aac(6')-Il / blaOXA-2 / blaIMP-11	mrkB	1..84930	84930
	-	inside	IScluster/Tn	sul1 / qacE / aac(6')-Il / blaOXA-2 / blaIMP-11	-	8522..37592	29070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-87)

Antitoxin

• Protein of unknown function DUF5397 (3-63)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10523.96 Da
• Isoelectric Point: 8.5263

>T35515 WP_096865196.1 NZ_AP022376:10092-10373 [Providencia rettgeri]
MYAISFDLVVAETSANHPKGVSQAYIDIATTLGNFGFQRVQGSLYTNHNEDMANLFNAMTALKAMSWFPKSVRDIRAFRI
EQWSDFTKTIKTP

Nucleotide

• Length: 282 bp

>T35515 NZ_AP022376:10092-10373 [Providencia rettgeri]
ATGTATGCAATTTCATTTGATTTAGTAGTAGCAGAGACATCAGCAAACCACCCTAAAGGTGTATCGCAAGCTTACATAGA
CATAGCTACAACACTGGGAAACTTCGGATTTCAACGAGTTCAAGGTAGCTTATACACAAACCACAACGAAGATATGGCTA
ACTTATTCAATGCAATGACCGCATTAAAAGCAATGAGTTGGTTTCCAAAGTCAGTAAGAGATATTAGAGCATTTAGAATT
GAACAATGGTCAGACTTCACTAAGACAATAAAAACGCCATAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6972.98 Da
• Isoelectric Point: 4.2850

>AT35515 WP_048609183.1 NZ_AP022376:9898-10089 [Providencia rettgeri]
MMTINKIPTGAIKTFGNYGVLYQVGEKDSDLPDGDILVNITLLESGEKEKYKLSQLIEDPEAK

Nucleotide

• Length: 192 bp

>AT35515 NZ_AP022376:9898-10089 [Providencia rettgeri]
ATGATGACAATAAACAAAATACCAACTGGAGCAATAAAAACCTTTGGGAACTATGGGGTTCTATATCAGGTTGGCGAGAA
AGATAGCGATCTTCCAGACGGAGATATTTTAGTGAATATCACCCTGCTAGAAAGTGGCGAAAAAGAGAAGTATAAATTAT
CTCAACTCATAGAAGATCCAGAAGCTAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0U3UH28