Detailed information of TA system TA035499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3069620..3070155
• Replicon: chromosome
• Accession: NZ_AP022373
• Organism: Providencia rettgeri strain BML2531

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: G7Y73_RS14145
• Protein ID: WP_164565364.1
• Coordinates: 3069847..3070155 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D4BV21
• Locus tag: G7Y73_RS14140
• Protein ID: WP_004255691.1
• Coordinates: 3069620..3069847 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y73_RS14115	3064803..3065105	+	303	WP_004255680.1	flagellar biosynthesis anti-sigma factor FlgM	-
G7Y73_RS14120	3065115..3065555	+	441	WP_004255682.1	flagellar export chaperone FlgN	-
G7Y73_RS14125	3065602..3065880	-	279	WP_004255684.1	helix-turn-helix domain-containing protein	-
G7Y73_RS14130	3066113..3068212	-	2100	WP_004255687.1	flagellar biosynthesis protein FlhA	-
G7Y73_RS14135	3068205..3069356	-	1152	WP_004255688.1	flagellar type III secretion system protein FlhB	-
G7Y73_RS14140	3069620..3069847	+	228	WP_004255691.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G7Y73_RS14145	3069847..3070155	+	309	WP_164565364.1	CcdB family protein	Toxin
G7Y73_RS14150	3070171..3070728	+	558	WP_144141262.1	hypothetical protein	-
G7Y73_RS14155	3070771..3071004	-	234	WP_080544466.1	helix-turn-helix domain-containing protein	-
G7Y73_RS14160	3071175..3071813	-	639	WP_094961165.1	protein phosphatase CheZ	-
G7Y73_RS14165	3071838..3072230	-	393	WP_004255705.1	chemotaxis response regulator CheY	-
G7Y73_RS14170	3072272..3073339	-	1068	WP_094961164.1	chemotaxis response regulator protein-glutamate methylesterase	-
G7Y73_RS14175	3073339..3074175	-	837	WP_004255713.1	chemotaxis protein-glutamate O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (5-75)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11745.81 Da
• Isoelectric Point: 8.0469

>T35499 WP_164565364.1 NZ_AP022373:3069847-3070155 [Providencia rettgeri]
MQYRLYQNRDDAIKYPYLLDIQSNTIDLLNTRLVIPLFDSRLVKKPLPARLTPQLVINGQVFILMTHQMACVPHSLLGKE
IVDLSSQRDTIKHAIDLLIDGF

Nucleotide

• Length: 309 bp

>T35499 NZ_AP022373:3069847-3070155 [Providencia rettgeri]
ATGCAATATCGCCTCTATCAAAACCGAGATGATGCTATCAAATACCCATATTTACTGGATATTCAAAGTAATACTATTGA
TTTATTGAATACTCGCTTAGTCATCCCGTTATTCGATAGCAGGCTAGTTAAAAAGCCATTACCCGCTCGCTTAACCCCCC
AATTAGTTATCAACGGGCAGGTTTTTATTTTAATGACTCACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTAAGTAGCCAACGAGACACCATTAAACACGCAATTGACTTACTGATTGATGGCTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8688.92 Da
• Isoelectric Point: 8.9375

>AT35499 WP_004255691.1 NZ_AP022373:3069620-3069847 [Providencia rettgeri]
MNRTKKTVSVTVAPELYEQARRAKLNLSAILACALQAELKRLEAQKWKNDNVEGFNELNRINEEYGLLSDKYKDF

Nucleotide

• Length: 228 bp

>AT35499 NZ_AP022373:3069620-3069847 [Providencia rettgeri]
ATGAATAGAACAAAAAAAACCGTCAGTGTCACTGTCGCGCCTGAACTCTACGAACAAGCAAGGCGGGCTAAACTTAACTT
ATCAGCAATACTGGCTTGTGCCTTACAAGCTGAATTAAAACGCCTCGAAGCGCAAAAATGGAAAAATGACAATGTCGAAG
GCTTTAATGAGTTAAACAGGATAAATGAAGAATATGGATTACTCTCAGATAAATACAAGGATTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G6Q922