Detailed information of TA system TA035494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4135919..4136454
• Replicon: chromosome
• Accession: NZ_AP022372
• Organism: Providencia rettgeri strain BML2526

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: pBML2531_RS18910
• Protein ID: WP_144141264.1
• Coordinates: 4136146..4136454 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: D4BV21
• Locus tag: pBML2531_RS18905
• Protein ID: WP_004255691.1
• Coordinates: 4135919..4136146 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
pBML2531_RS18885	4131202..4131642	+	441	WP_004255682.1	flagellar export chaperone FlgN	-
pBML2531_RS18890	4132044..4132259	+	216	WP_094961170.1	hypothetical protein	-
pBML2531_RS18895	4132412..4134511	-	2100	WP_004255687.1	flagellar biosynthesis protein FlhA	-
pBML2531_RS18900	4134504..4135655	-	1152	WP_140170721.1	flagellar type III secretion system protein FlhB	-
pBML2531_RS18905	4135919..4136146	+	228	WP_004255691.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
pBML2531_RS18910	4136146..4136454	+	309	WP_144141264.1	CcdB family protein	Toxin
pBML2531_RS18915	4136470..4137027	+	558	WP_164455776.1	hypothetical protein	-
pBML2531_RS18920	4137070..4137303	-	234	WP_080544466.1	helix-turn-helix domain-containing protein	-
pBML2531_RS18925	4137474..4138112	-	639	WP_094961165.1	protein phosphatase CheZ	-
pBML2531_RS18930	4138137..4138529	-	393	WP_004255705.1	chemotaxis response regulator CheY	-
pBML2531_RS18935	4138571..4139638	-	1068	WP_094961164.1	chemotaxis response regulator protein-glutamate methylesterase	-
pBML2531_RS18940	4139638..4140474	-	837	WP_164456333.1	chemotaxis protein-glutamate O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (5-75)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11757.87 Da
• Isoelectric Point: 8.0469

>T35494 WP_144141264.1 NZ_AP022372:4136146-4136454 [Providencia rettgeri]
MQYRLYQNRDDAIKYPYLLDIQSNIIDLLNTRLVIPLFDSRLVKKPLPARLTPQLVINGQVFILMTHQMACVPHSLLGKE
IVDLSSQRDTIKHAIDLLIDGF

Nucleotide

• Length: 309 bp

>T35494 NZ_AP022372:4136146-4136454 [Providencia rettgeri]
ATGCAATATCGCCTCTATCAAAACCGAGATGATGCTATCAAATACCCATATTTACTGGATATTCAAAGTAATATTATTGA
TTTATTGAATACTCGCTTAGTCATCCCGTTATTCGATAGCAGGCTAGTTAAAAAGCCATTACCCGCTCGCTTAACCCCCC
AATTAGTTATCAACGGGCAGGTTTTTATTTTAATGACTCACCAAATGGCTTGCGTCCCTCATTCATTATTAGGGAAAGAA
ATCGTTGACTTAAGTAGCCAACGAGACACCATTAAACACGCAATTGACTTACTGATTGATGGCTTTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8688.92 Da
• Isoelectric Point: 8.9375

>AT35494 WP_004255691.1 NZ_AP022372:4135919-4136146 [Providencia rettgeri]
MNRTKKTVSVTVAPELYEQARRAKLNLSAILACALQAELKRLEAQKWKNDNVEGFNELNRINEEYGLLSDKYKDF

Nucleotide

• Length: 228 bp

>AT35494 NZ_AP022372:4135919-4136146 [Providencia rettgeri]
ATGAATAGAACAAAAAAAACCGTCAGTGTCACTGTCGCGCCTGAACTCTACGAACAAGCAAGGCGGGCTAAACTTAACTT
ATCAGCAATACTGGCTTGTGCCTTACAAGCTGAATTAAAACGCCTCGAAGCGCAAAAATGGAAAAATGACAATGTCGAAG
GCTTTAATGAGTTAAACAGGATAAATGAAGAATATGGATTACTCTCAGATAAATACAAGGATTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G6Q922