Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1314232..1314850 Replicon chromosome
Accession NZ_AP022362
Organism Escherichia coli strain E302

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag G6M96_RS06370 Protein ID WP_001291435.1
Coordinates 1314232..1314450 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag G6M96_RS06375 Protein ID WP_000344800.1
Coordinates 1314476..1314850 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M96_RS06335 1309519..1310091 + 573 WP_000779831.1 YbaY family lipoprotein -
G6M96_RS06340 1310122..1310433 - 312 WP_000409908.1 MGMT family protein -
G6M96_RS06350 1310812..1311165 + 354 WP_000878135.1 DUF1428 family protein -
G6M96_RS06355 1311207..1312757 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
G6M96_RS06360 1312921..1313391 - 471 WP_000136192.1 YlaC family protein -
G6M96_RS06365 1313507..1314058 - 552 WP_000102539.1 maltose O-acetyltransferase -
G6M96_RS06370 1314232..1314450 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
G6M96_RS06375 1314476..1314850 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
G6M96_RS06380 1315396..1318545 - 3150 WP_163409485.1 efflux RND transporter permease AcrB -
G6M96_RS06385 1318568..1319761 - 1194 Protein_1247 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35455 WP_001291435.1 NZ_AP022362:c1314450-1314232 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35455 NZ_AP022362:c1314450-1314232 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35455 WP_000344800.1 NZ_AP022362:c1314850-1314476 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35455 NZ_AP022362:c1314850-1314476 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References