Detailed information of TA system TA035451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 700863..701683
• Replicon: chromosome
• Accession: NZ_AP022362
• Organism: Escherichia coli strain E302

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: G6M96_RS03475
• Protein ID: WP_001054379.1
• Coordinates: 701426..701683 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: G6M96_RS03470
• Protein ID: WP_000123957.1
• Coordinates: 700863..701414 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6M96_RS03445	696557..697870	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
G6M96_RS03450	697882..698160	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
G6M96_RS03455	698157..699278	-	1122	WP_000010833.1	M42 family metallopeptidase	-
G6M96_RS25730	699523..699639	-	117	Protein_670	VOC family protein	-
G6M96_RS03460	699677..699937	-	261	WP_000077645.1	hypothetical protein	-
G6M96_RS03465	700065..700811	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
G6M96_RS03470	700863..701414	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
G6M96_RS03475	701426..701683	-	258	WP_001054379.1	YjhX family toxin	Toxin
G6M96_RS03480	702060..702224	-	165	Protein_675	GNAT family N-acetyltransferase	-
G6M96_RS03485	702257..703402	-	1146	Protein_676	IS3-like element ISEc52 family transposase	-
G6M96_RS03490	703465..704571	+	1107	Protein_677	DNA helicase	-
G6M96_RS03495	705154..706134	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T35451 WP_001054379.1 NZ_AP022362:c701683-701426 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35451 NZ_AP022362:c701683-701426 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT35451 WP_000123957.1 NZ_AP022362:c701414-700863 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT35451 NZ_AP022362:c701414-700863 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030