Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 140527..141052 Replicon chromosome
Accession NZ_AP022362
Organism Escherichia coli strain E302

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag G6M96_RS00725 Protein ID WP_001159868.1
Coordinates 140747..141052 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag G6M96_RS00720 Protein ID WP_000813634.1
Coordinates 140527..140745 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M96_RS00695 135811..137424 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
G6M96_RS00700 137453..137800 - 348 WP_047626551.1 IS66 family insertion sequence element accessory protein TnpB -
G6M96_RS00705 137797..138222 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
G6M96_RS00710 138284..139435 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
G6M96_RS00715 139469..139981 - 513 WP_000151784.1 hypothetical protein -
G6M96_RS00720 140527..140745 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
G6M96_RS00725 140747..141052 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
G6M96_RS00730 141053..141859 + 807 WP_000016982.1 site-specific integrase -
G6M96_RS00735 142633..143385 + 753 WP_163409461.1 replication initiation protein RepE -
G6M96_RS00740 143973..145139 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sitABCD / mph(A) iutA / iucD / iucC / iucC / iucB / iucA 86670..205797 119127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T35448 WP_001159868.1 NZ_AP022362:140747-141052 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T35448 NZ_AP022362:140747-141052 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT35448 WP_000813634.1 NZ_AP022362:140527-140745 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT35448 NZ_AP022362:140527-140745 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References