Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 133406..134049 Replicon chromosome
Accession NZ_AP022362
Organism Escherichia coli strain E302

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag G6M96_RS00675 Protein ID WP_001034046.1
Coordinates 133406..133822 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag G6M96_RS00680 Protein ID WP_001261278.1
Coordinates 133819..134049 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M96_RS00660 128543..128959 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
G6M96_RS00665 128956..129186 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
G6M96_RS00670 129567..133361 + 3795 WP_001144732.1 hypothetical protein -
G6M96_RS00675 133406..133822 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
G6M96_RS00680 133819..134049 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
G6M96_RS00685 134314..134814 + 501 WP_000528932.1 hypothetical protein -
G6M96_RS00690 134827..135600 + 774 WP_000905949.1 hypothetical protein -
G6M96_RS00695 135811..137424 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
G6M96_RS00700 137453..137800 - 348 WP_047626551.1 IS66 family insertion sequence element accessory protein TnpB -
G6M96_RS00705 137797..138222 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sitABCD / mph(A) iutA / iucD / iucC / iucC / iucB / iucA 86670..205797 119127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T35447 WP_001034046.1 NZ_AP022362:c133822-133406 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T35447 NZ_AP022362:c133822-133406 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT35447 WP_001261278.1 NZ_AP022362:c134049-133819 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT35447 NZ_AP022362:c134049-133819 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References