35445

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 98474..99033 Replicon chromosome
Accession NZ_AP022362
Organism Escherichia coli strain E302

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag G6M96_RS00510 Protein ID WP_000421272.1
Coordinates 98758..99033 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag G6M96_RS00505 Protein ID WP_001387467.1
Coordinates 98474..98758 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M96_RS00480 93937..94377 - 441 Protein_95 zinc-binding dehydrogenase -
G6M96_RS00485 94778..95668 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
G6M96_RS00490 95693..96073 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
G6M96_RS00495 96106..97071 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
G6M96_RS00500 97117..97869 - 753 WP_000562172.1 IclR family transcriptional regulator -
G6M96_RS00505 98474..98758 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
G6M96_RS00510 98758..99033 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G6M96_RS00515 99139..99432 + 294 WP_001105060.1 hypothetical protein -
G6M96_RS00520 99467..99595 - 129 Protein_103 IS481 family transposase -
G6M96_RS00525 99628..100797 - 1170 WP_001072355.1 hypothetical protein -
G6M96_RS00530 101290..101597 + 308 Protein_105 type II toxin-antitoxin system ParD family antitoxin -
G6M96_RS00535 101940..102122 + 183 WP_042344623.1 hypothetical protein -
G6M96_RS00540 102243..102983 + 741 WP_001066953.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element sitABCD / mph(A) iutA / iucD / iucC / iucC / iucB / iucA 86670..205797 119127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T35445 WP_000421272.1 NZ_AP022362:98758-99033 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T35445 NZ_AP022362:98758-99033 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT35445 WP_001387467.1 NZ_AP022362:98474-98758 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT35445 NZ_AP022362:98474-98758 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References