Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1353748..1354366 Replicon chromosome
Accession NZ_AP022351
Organism Escherichia coli strain E138

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GR930_RS06515 Protein ID WP_001291435.1
Coordinates 1353748..1353966 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GR930_RS06520 Protein ID WP_000344800.1
Coordinates 1353992..1354366 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GR930_RS06480 1349036..1349608 + 573 WP_000779841.1 YbaY family lipoprotein -
GR930_RS06485 1349639..1349950 - 312 WP_000409908.1 MGMT family protein -
GR930_RS06495 1350329..1350682 + 354 WP_000878147.1 DUF1428 family protein -
GR930_RS06500 1350724..1352274 - 1551 WP_001545832.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GR930_RS06505 1352438..1352908 - 471 WP_000136192.1 YlaC family protein -
GR930_RS06510 1353024..1353575 - 552 WP_000102539.1 maltose O-acetyltransferase -
GR930_RS06515 1353748..1353966 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
GR930_RS06520 1353992..1354366 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GR930_RS06525 1354912..1358061 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
GR930_RS06530 1358084..1359277 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35419 WP_001291435.1 NZ_AP022351:c1353966-1353748 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35419 NZ_AP022351:c1353966-1353748 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35419 WP_000344800.1 NZ_AP022351:c1354366-1353992 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35419 NZ_AP022351:c1354366-1353992 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References