Detailed information of TA system TA035403

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1500970..1501571
• Replicon: chromosome
• Accession: NZ_AP022347
• Organism: Fluviibacter phosphoraccumulans strain ICHIJ1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ICHIJ1_RS07495
• Protein ID: WP_162050014.1
• Coordinates: 1501266..1501571 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICHIJ1_RS07490
• Protein ID: WP_162050013.1
• Coordinates: 1500970..1501269 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIJ1_RS07460	1496639..1497328	+	690	WP_174237203.1	electron transport complex subunit E	-
ICHIJ1_RS07465	1497341..1498000	+	660	WP_162050009.1	endonuclease III	-
ICHIJ1_RS07470	1498017..1498439	+	423	WP_162050942.1	DUF1841 family protein	-
ICHIJ1_RS07475	1498521..1499258	-	738	WP_162050010.1	3-ketoacyl-ACP reductase	-
ICHIJ1_RS07480	1499413..1500630	+	1218	WP_162050011.1	MFS transporter	-
ICHIJ1_RS07485	1500635..1500946	+	312	WP_162050012.1	hypothetical protein	-
ICHIJ1_RS07490	1500970..1501269	-	300	WP_162050013.1	putative addiction module antidote protein	Antitoxin
ICHIJ1_RS07495	1501266..1501571	-	306	WP_162050014.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIJ1_RS07500	1501841..1502062	-	222	WP_202930672.1	DUF4224 domain-containing protein	-
ICHIJ1_RS07505	1502297..1502572	-	276	WP_162050015.1	hypothetical protein	-
ICHIJ1_RS07510	1502918..1503583	+	666	WP_162050016.1	hypothetical protein	-
ICHIJ1_RS07520	1504963..1505307	-	345	WP_202930671.1	DUF86 domain-containing protein	-
ICHIJ1_RS07525	1505304..1505594	-	291	WP_162050018.1	nucleotidyltransferase family protein	-
ICHIJ1_RS07535	1505787..1506107	-	321	WP_162050019.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-92)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11439.08 Da
• Isoelectric Point: 9.9574

>T35403 WP_162050014.1 NZ_AP022347:c1501571-1501266 [Fluviibacter phosphoraccumulans]
MKLIYTTEIFDAWFGRLKDKEVARRIQIRIDRAEDGHFGDCKSVGGGVSEMRIHHGPGYRVYFMQRGFEVVILLAGGDKS
SQSRDIQTAIHIAQQIKGSRS

Nucleotide

• Length: 306 bp

>T35403 NZ_AP022347:c1501571-1501266 [Fluviibacter phosphoraccumulans]
ATGAAACTTATCTATACGACAGAAATTTTTGATGCTTGGTTTGGCCGATTAAAAGACAAAGAAGTTGCGCGGCGGATTCA
GATTCGGATCGACAGAGCAGAAGATGGTCATTTCGGAGACTGTAAGTCTGTTGGTGGCGGTGTTTCAGAAATGCGAATTC
ATCATGGGCCGGGATATCGGGTGTATTTCATGCAACGAGGATTCGAGGTGGTGATTTTACTAGCAGGTGGTGACAAGTCA
TCACAGTCCAGAGATATTCAAACAGCTATACATATAGCTCAGCAGATTAAGGGTAGCAGATCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10721.36 Da
• Isoelectric Point: 7.2776

>AT35403 WP_162050013.1 NZ_AP022347:c1501269-1500970 [Fluviibacter phosphoraccumulans]
MKAVKLRRWDSAEHLKTSEEMALYLEACFQEAGDDAAFIAKALGNIARAKGMSQLAQETGMGRESLYKALSGEGNPSFAT
ILKVTRALGIELHAMPVHA

Nucleotide

• Length: 300 bp

>AT35403 NZ_AP022347:c1501269-1500970 [Fluviibacter phosphoraccumulans]
ATGAAAGCAGTAAAGCTACGTAGATGGGATAGCGCCGAACATCTGAAAACATCAGAAGAGATGGCGTTGTATCTAGAAGC
TTGCTTCCAAGAAGCAGGTGATGATGCTGCATTTATTGCCAAAGCACTGGGTAACATTGCACGTGCCAAAGGCATGTCAC
AGCTAGCCCAAGAGACCGGTATGGGACGGGAGAGTCTTTATAAAGCGCTCTCCGGAGAAGGTAACCCAAGCTTTGCAACC
ATTCTCAAGGTGACACGTGCTTTAGGTATTGAGCTTCATGCGATGCCAGTTCATGCCTAG