Detailed information of TA system TA035402

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1341000..1341601
• Replicon: chromosome
• Accession: NZ_AP022347
• Organism: Fluviibacter phosphoraccumulans strain ICHIJ1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ICHIJ1_RS06750
• Protein ID: WP_162049885.1
• Coordinates: 1341299..1341601 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICHIJ1_RS06745
• Protein ID: WP_162049884.1
• Coordinates: 1341000..1341299 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICHIJ1_RS06740	1337843..1340980	-	3138	WP_162049883.1	N-6 DNA methylase	-
ICHIJ1_RS06745	1341000..1341299	-	300	WP_162049884.1	putative addiction module antidote protein	Antitoxin
ICHIJ1_RS06750	1341299..1341601	-	303	WP_162049885.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICHIJ1_RS06760	1341934..1343340	+	1407	WP_162049886.1	glutamate--tRNA ligase	-
ICHIJ1_RS06780	1343924..1345387	+	1464	WP_162049887.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-95)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11535.29 Da
• Isoelectric Point: 8.9004

>T35402 WP_162049885.1 NZ_AP022347:c1341601-1341299 [Fluviibacter phosphoraccumulans]
MKIIYTTKIFDSWYQKLKDREAARFIQVRIDRAEDGNFGDCKPIGEGVSEMRLHVGPGYRLYFMQQGLEIVILLAGGNKS
SQSRDIEIALQIAREIRENK

Nucleotide

• Length: 303 bp

>T35402 NZ_AP022347:c1341601-1341299 [Fluviibacter phosphoraccumulans]
ATGAAAATCATTTATACAACGAAGATCTTTGATTCTTGGTATCAAAAGCTCAAAGATCGGGAAGCGGCTCGGTTCATTCA
GGTGCGAATTGATCGCGCAGAAGATGGCAACTTTGGTGATTGCAAGCCCATTGGTGAAGGCGTATCTGAAATGCGTTTGC
ATGTAGGGCCAGGCTACCGTCTGTATTTTATGCAACAAGGTTTAGAGATTGTGATTTTGCTGGCAGGCGGAAACAAATCG
TCACAGAGCCGAGATATAGAGATTGCTTTGCAAATTGCAAGAGAGATTAGGGAGAACAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10681.28 Da
• Isoelectric Point: 6.7170

>AT35402 WP_162049884.1 NZ_AP022347:c1341299-1341000 [Fluviibacter phosphoraccumulans]
MKTIQLRRWDSAEHLKTPEDMALYLEACLQEAGDDAAFVAKALGNIARAKGMSQLANETGMGRESLYKALSGEGNPSFAT
ILKVTRALGIELHALPAHV

Nucleotide

• Length: 300 bp

>AT35402 NZ_AP022347:c1341299-1341000 [Fluviibacter phosphoraccumulans]
ATGAAAACCATTCAGTTACGTCGCTGGGATAGTGCCGAACACCTTAAAACGCCGGAGGATATGGCGTTATATCTCGAGGC
TTGTCTCCAAGAAGCGGGAGACGACGCAGCATTTGTAGCCAAAGCACTAGGTAATATCGCACGCGCCAAAGGCATGTCGC
AGCTTGCTAATGAAACAGGTATGGGGCGTGAAAGTCTTTATAAAGCGCTATCGGGTGAAGGTAACCCAAGTTTTGCCACG
ATCCTTAAGGTTACCCGTGCGCTCGGCATTGAGCTGCATGCTCTGCCAGCTCACGTCTAA